Lipolysis Assay Kit (Colorimetric) (ab185433)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell culture supernatant
- Sensitivity: 0.2 nmol/well
Overview
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Product name
Lipolysis Assay Kit (Colorimetric)
See all Lipolysis kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant -
Assay type
Quantitative -
Sensitivity
< 0.2 nmol/well -
Range
2 nmol/well - 10 nmol/well -
Assay time
0h 40m -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Abcam's Lipolysis Assay Kit (Colorimetric) (ab185433) is simple and easy-to-use. The assay measures glycerol released from 3T3-L1 cells after induction of lipolysis using colorimetric method. The color intensity is directly proportional to the amount of glycerol. This assay kit can detect less than 200 pmol of glycerol.
Lipolysis assay protocol summary:
- add samples and standards to wells
- add reaction mix
- incubate for 30 min
- analyze with microplate reader -
Notes
This product is manufactured by BioVision, an Abcam company and was previously called K577 Lipolysis (3T3-L1) Colorimetric Assay Kit. K577-100 is the same size as the 100 test size of ab185433.
Lipolysis is the hydrolysis of triglycerides within the cell into glycerol and free fatty acids. The glycerol and free fatty acids are then released into the bloodstream or culture media. Lipolysis occurs in essentially all cells, but is most abundant in white and brown adipose tissue. Deficiencies in lipolysis lead to increased intracellular lipid accumulation, resulting in abnormal cellular physiology, hyperlipidemia, and insulin resistance. Lipolysis can be induced by catecholamine and certain hormones. The kit includes synthetic catecholamine, Isoproterenol, which activates β-adrenergic receptors. This leads to activation of adenylate cyclase, which catalyzes the conversion of ATP to cAMP. cAMP then serves as a second messenger to activate hormone-sensitive lipase, which hydrolyzes the triglycerides. This pathway can be inhibited by insulin.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Assay Buffer V 1 x 25ml Enzyme Mix VI 1 vial Glycerol Standard 1 x 0.2ml Isoproterenol 1 x 50µl Buffer IV 1 x 17ml Wash Buffer VIII 1 x 22ml OxiRed Probe 1 x 0.2ml -
Research areas
Associated products
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Related Products
Images
Datasheets and documents
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SDS download
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Datasheet download
References (14)
ab185433 has been referenced in 14 publications.
- Christen L et al. Myoglobin-mediated lipid shuttling increases adrenergic activation of brown and white adipocyte metabolism and is as a marker of thermogenic adipocytes in humans. Clin Transl Med 12:e1108 (2022). PubMed: 36480426
- Reddy Sankaran K et al. A bioactive fraction of Pterocarpus santalinus inhibits adipogenesis and inflammation in 3T3-L1 cells via modulation of PPAR-?/SREBP-1c and TNF-a/IL-6. 3 Biotech 11:233 (2021). PubMed: 33968577
- Molinari F et al. SIRT5 Inhibition Induces Brown Fat-Like Phenotype in 3T3-L1 Preadipocytes. Cells 10:N/A (2021). PubMed: 34066961
- Karunakaran RS et al. Anti-Obesity and Lipid Lowering Activity of Bauhiniastatin-1 is Mediated Through PPAR-?/AMPK Expressions in Diet-Induced Obese Rat Model. Front Pharmacol 12:704074 (2021). PubMed: 34366856
- Goddi A et al. Laminin-a4 Is Upregulated in Both Human and Murine Models of Obesity. Front Endocrinol (Lausanne) 12:698621 (2021). PubMed: 34394003