Methylglyoxal Assay Kit (Fluorometric) (ab273284)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate
- Sample type: Cell Lysate, Other biological fluids, Tissue Homogenate
Overview
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Product name
Methylglyoxal Assay Kit (Fluorometric)
See all Methylglyoxal kits -
Detection method
Fluorescent -
Sample type
Other biological fluids, Cell Lysate, Tissue Homogenate -
Assay type
Quantitative -
Assay duration
Multiple steps standard assay -
Product overview
Methylglyoxal (MG) Assay Activity Kit (Fluorometric) (ab273284) can detect MG using an enzyme-coupled reaction which reduces the fluorogenic probe. The reduced fluorophore produces a stable signal (Ex/Em= 535/587nm), which is directly proportional to the amount of MG in samples.
The assay is simple, reproducible and can specifically detect as low as 6 pmol of MG in a 100 µl reaction.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K461 PicoProbeTM Methylglyoxal Assay Kit (Fluorometric). K461-100 is the same size as the 100 test size of ab273284.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Developer XI 1 vial Enzyme Mix A 1 x 22µl Enzyme Mix B 1 x 120µl Enzyme Mix XIII 1 vial Extraction Solution 1 x 2ml GSH Standard 1 vial MG Assay Buffer I 1 x 25ml PicoProbe I 1 x 0.4ml Substrate Mix A 1 x 1.1ml -
Research areas
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Relevance
Methylglyoxal, also called pyruvaldehyde or 2-oxo-propanal (CH3-CO-CH=O or C3H4O2) is the aldehyde form of pyruvic acid. Methylglyoxal is both an aldehyde and a ketone. In the body, methylglyoxal may form from 3-amino acetone, which is an intermediate of threonine catabolism. This compound, as well as glyoxal, is a byproduct of metabolism, but cannot be used. The aldehyde reacts with glutathione, a tripeptide consisting of glutamic acid, cysteine, and glycine. Glutathione reduces methylglyoxal to 1-hydroxy acetone, a ketol. 1-Hydroxy acetone goes through enediol tautomerism to lactaldehyde. Then the lactaldehyde is oxidised by glutathione to D-lactic acid. Methylglyoxal is also called a ketal, because it has an aldehydic and ketonic carbonyl group. It is a type of the advanced glycation endproducts (AGEs) resulting from sugar modifications, such as the Maillard reaction. When the reductone is cleaved, pyruvaldehyde forms. -
Alternative names
- 2 oxo propanal
- MG
- pyruvaldehyde
Images
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Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
MG Standard Curve, results from multiple experiments
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Example data.
MG activity was measured in Jurkat cell ysates (15 µg lysates). All assays were performed following kit protocol.
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Example data.
MG activity was measured for mouse liver (4 mg tissue), rat brain (12 mg tissue) and human cerebrospinal fluid (CSF, 2 µl).
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab273284 has not yet been referenced specifically in any publications.