Nuclear/Cytosol Fractionation Kit (ab289882)
Key features and details
- Sample type: Adherent cells, Suspension cells, Tissue
Overview
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Product name
Nuclear/Cytosol Fractionation Kit -
Sample type
Tissue, Adherent cells, Suspension cells -
Species reactivity
Reacts with: Mammals -
Product overview
This Nuclear/Cytosol Extraction Kit (ab289882, K266) provides a complete system that enables the separation of nuclear extract from the cytoplasmic fraction of mammalian cells. The optimized reagents and procedures provided with the kit allow separation of nuclear and cytoplasmic fractions quickly with little or no cross-contaminations. The extracted nuclear and cytoplasmic protein fractions are functional and compatible with downstream assays such as transcriptional activity, RNA splicing, gel shift assay, reporter assays, enzyme activity assays, and Western blotting.
- Simple procedure; takes less than 2-3 hours
- The optimized reagents and procedures provided with the kit allow separation of nuclear and cytoplasmic extracts from cells relatively quick with little or no cross-contaminations
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Extraction Buffer VI 1 x 20ml Lysis Buffer X 1 x 1.2ml DTT II 1 x 100µl Extraction Buffer V 1 x 10ml Protease Inhibitor Cocktail I 1 vial -
Research areas
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Relevance
Nuclear/Cytosol Fractionation is a method that enables the separation of the nuclear extract from the cytoplasmic fraction of mammalian cells.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab289882 has not yet been referenced specifically in any publications.