DPPH Antioxidant Assay Kit (Colorimetric) (ab289847)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate
- Sample type: Food samples, Other biological fluids, Serum
Overview
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Product name
DPPH Antioxidant Assay Kit (Colorimetric) -
Detection method
Colorimetric -
Sample type
Serum, Other biological fluids, Food samples -
Assay type
Quantitative -
Assay duration
Multiple steps standard assay -
Product overview
This DPPH Antioxidant Assay Kit (ab289847, K2078) is a high-throughput adaptable, microplate-based assay that allows the rapid quantification of antioxidant capacity of many samples including foods, beverages, biological fluids (e.g. human serum), etc. In this assay, DPPH free radical, which is deep blue in color abstracts a hydrogen atom in a one-electron reaction to form 2,2-diphenyl-1-picrylhydrazine (DPPH-H), which is pale yellow in color. The color change is associated with a decrease in absorbance at 517 nm. The decrease in absorbance is proportional to the antioxidant capacity of the sample. The synthetic antioxidant, Trolox is included in the kit and is used to standardize the sample antioxidant capacity relative to Trolox (Trolox Equivalents Antioxidant Capacity, TEAC).
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 100 tests DPPH Probe 1 vial DPPH Assay Buffer 1 x 50ml Trolox Standard 1 vial
Images
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Linear relationship between DPPH concentrations and Absorbance.
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Trolox Standard Curve (linear range of inhibition changes after Trolox treatment under 300 µM DPPH concentration).
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DPPH assay using green tea and kiwi fruit samples.
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Logistic fitting including the dilution factor for calculating EC50.
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Logistic fitting including the dilution factor for calculating EC50.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab289847 has not yet been referenced specifically in any publications.