Human Platelet Isolation Kit (ab289839)
Key features and details
- Sample type: Whole Blood
Overview
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Product name
Human Platelet Isolation Kit -
Sample type
Whole Blood -
Species reactivity
Reacts with: Human -
Product overview
This Human Platelet Isolation Kit (ab289839, K2071) allows the in vitro isolation of intact, viable, unactivated platelets. The kit enables the high recovery of platelets of (≥ 1 x107 platelets/ml) yielding approximately 80% of the total platelets present in 1 ml of whole blood. The viability stain included in the kit is used to identify living platelets indicating that >90% of the platelets in the isolated fraction is viable. The isolated fraction contains >95% platelets and has minimal contamination of red blood cells and leukocytes.
Detection Method: Density gradient based platelet isolation
Sample Types: Fresh whole blood collected < 8 hrs prior to platelet isolation, with EDTA or citrate anti-coagulants.
Applications:
- Isolation of platelets from whole blood.
- Determination of platelet viability, purity, and quality.
- Studying platelet activation and characterization of platelet morphology.
- In vitro assays to evaluate primary platelet functions such as clotting, chemokine release, adhesion, chemotaxis etc.
- Studying platelet surface proteins.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 25 tests BSA Solution 1 x 5ml Cell Dye II 1 vial Density Gradient Media II 1 x 25ml Gradient Dilution Buffer I 1 x 110ml Platelet Storage Buffer 2 x 50ml -
Research areas
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Relevance
Like red blood cells, platelets are anuclear (no cell nucleus) and discoid (disc shaped); they measure 1.5 to 3.0 µm in diameter. The body has a very limited reserve of platelets, so they can be rapidly depleted. They contain RNA, a canalicular system, and several different types of granules; lysosomes (containing acid hydrolases), dense bodies (containing ADP, ATP serotonin and calcium) and alpha granules (containing fibrinogen, factor V, vitronectin, thrombospondin and von Willebrand factor), the contents of which are released upon activation of the platelet. These granule contents play an important role in both hemostasis and in the inflammatory response. Platelets or thrombocytes are the cell fragments circulating in the blood that are involved in the cellular mechanisms of primary hemostasis leading to the formation of blood clots. Dysfunction or low levels of platelets predisposes to bleeding, while high levels, although usually asymptomatic, may increase the risk of thrombosis. Platelets are produced in the bone marrow; the progenitor cell for platelets is the megakaryocyte. It is about twelve times larger than an erythrocyte, possesses a lobed nucleus and sheds platelets into the circulation. -
Cellular localization
Cell Membrane
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab289839 has not yet been referenced specifically in any publications.