DNAse I Activity Assay Kit (Fluorometric)

High-throughput compatible, quick and easy screening for DNAse activity in biological samples; Includes DNAse positive control
Catalog #: K429 | abID: ab234056

Product Details

abID ab234056
Cat # +Size K429-100
Size 100 assays
Kit Summary • Detection method - Fluorescence (Ex/Em 651/681 nm)
• Species reactivity - Eukaryotes
• Applications - Measurement of DNAse activity in various biological samples.
Detection Method Fluorescence (Ex/Em 651/681 nm)
Species Reactivity Eukaryotes
Applications • Measurement of DNAse I activity of purified proteins
• Quantitative analysis of DNAse I mutants and inhibitors • Comparative examination of DNAse I activity in biological samples
Features & Benefits Quantitative analysis of DNAse I mutants and inhibitors
Kit Components • 10X DNAse I Assay Buffer
• DNA Probe
• DNA Probe Re-suspension Buffer
• DNAse I Positive Control
• Positive Control Re-suspension Buffer
• Molecular Biology Grade Water
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Deoxyribonuclease I (DNAse I) is an endonuclease that cleaves DNA phosphodiester bonds yielding 5′-phosphorylated and 3′-hydroxylated oligonucleotides. DNAse I targets single-stranded DNA, double-stranded DNA, and chromatin in a non-specific manner. As an important player in cellular waste management, DNAse I is normally secreted extracellularly to clear the system from circulating cell-free DNA, foreign DNA from food digestion or potential pathogens, and endogenous chromosomal DNA from apoptotic and necrotic cells. Abnormal DNAse I activity occurs in association with a variety of cancers and auto-immune illnesses that exhibit elevated levels of cell-free DNA. Furthermore, DNAse I has been therapeutically used in cystic fibrosis patients to degrade DNA and reduce sputum viscosity. BioVision’s DNAse I Activity Fluorometric Assay Kit allows for quantitative evaluation of DNAse I activity of purified enzymes and their inhibitors as well as comparative examination of DNAse I activity in biological samples. Enzyme activity is detected upon cleavage of a DNA Probe, which yields a fluorescent DNA product measured at Ex/Em = 651/681 nm. The limit of quantification (L.O.Q) is 178 fmoles of DNA probe cleaved per minute per ml

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Could you let me know how the DNA probe creates its fluorescence? Any general information about the structure of the probe would be appreciated.
The DNA probe is ~30 bp long , has a fluorescent dye attached to the 5` end and a fluorescent quencher attached to the 3` end. The fluorescence is generated when the probe is cleaved by DNase activity.