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Product Details
| Cat # +Size | K429-100 |
|---|---|
| Size | 100 assays |
| Kit Summary | • Detection method - Fluorescence (Ex/Em 651/681 nm) • Species reactivity - Eukaryotes • Applications - Measurement of DNAse activity in various biological samples. |
| Detection Method | Fluorescence (Ex/Em 651/681 nm) |
| Species Reactivity | Eukaryotes |
| Applications | • Measurement of DNAse I activity of purified proteins • Quantitative analysis of DNAse I mutants and inhibitors • Comparative examination of DNAse I activity in biological samples |
| Features & Benefits | Quantitative analysis of DNAse I mutants and inhibitors |
| Kit Components | • 10X DNAse I Assay Buffer • DNA Probe • DNA Probe Re-suspension Buffer • DNAse I Positive Control • Positive Control Re-suspension Buffer • Molecular Biology Grade Water |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
Deoxyribonuclease I (DNAse I) is an endonuclease that cleaves DNA phosphodiester bonds yielding 5′-phosphorylated and 3′-hydroxylated oligonucleotides. DNAse I targets single-stranded DNA, double-stranded DNA, and chromatin in a non-specific manner. As an important player in cellular waste management, DNAse I is normally secreted extracellularly to clear the system from circulating cell-free DNA, foreign DNA from food digestion or potential pathogens, and endogenous chromosomal DNA from apoptotic and necrotic cells. Abnormal DNAse I activity occurs in association with a variety of cancers and auto-immune illnesses that exhibit elevated levels of cell-free DNA. Furthermore, DNAse I has been therapeutically used in cystic fibrosis patients to degrade DNA and reduce sputum viscosity. BioVision’s DNAse I Activity Fluorometric Assay Kit allows for quantitative evaluation of DNAse I activity of purified enzymes and their inhibitors as well as comparative examination of DNAse I activity in biological samples. Enzyme activity is detected upon cleavage of a DNA Probe, which yields a fluorescent DNA product measured at Ex/Em = 651/681 nm. The limit of quantification (L.O.Q) is 178 fmoles of DNA probe cleaved per minute per ml
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Could you let me know how the DNA probe creates its fluorescence? Any general information about the structure of the probe would be appreciated.
The DNA probe is ~30 bp long , has a fluorescent dye attached to the 5` end and a fluorescent quencher attached to the 3` end. The fluorescence is generated when the probe is cleaved by DNase activity.
