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DNA Quantification Assay Kit (Fluorometric)

Sensitive, specific assay. Protein, RNA and ssDNA do not react with the probe
Catalog #: K539

In stock

SKU-Size Size Price Qty
K539-200 200 assays
$175.00
K539-2000 2000 assays
$295.00
More Sizes Get Quote

Product Details

Detection Method Fluorometric (Ex/Em = 492/528 nm)
Species Reactivity All
Applications Quantification of DNA for rtPCR, qPCR, Next Generation Sequencing (NGS)
Features & Benefits • Simple, one-step, results in 30 min or less
• Sensitive, specific
• Does not detect protein, RNA or ssDNA
Kit Components • 200X DNA Probe (in DMSO)
• λDNA Standard (3 ng/µl)
• 10X DNA Buffer
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

DNA quantification is essential for a variety of applications, such as real-time PCR (rtPCR), qPCR, Next Generation Sequencing (NGS), etc. The measurement of DNA using analytical instruments (i.e. “nanodrop”) is often limited by the presence/contamination of other macromolecules like protein and RNAs found in biological samples. However, the development of highly specific probes that bind to DNA allows the measurement of this macromolecule using a fluorimeter. The biggest advantages of this approach is protein, RNA and single-stranded DNA (ssDNA) are not detected. BioVision’s DNA Quantification Assay Kit provides a quick, specific, and easy method for the measurement of DNA concentrations in a wide variety of samples. In this assay, DNA specifically reacts with a probe producing a stable fluorescence signal (Ex/Em = 492/528 nm). The kit is simple to perform, specific, sensitive and high-throughput adaptable with a wide detection range (0.4-150 ng DNA) and a limit of detection of 4 pg/µl dsDNA in samples.


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