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DNA Methyltransferase 1 (Clone 60B1220.1) Antibody

Monoclonal antibody to detect DNMT1 in Human, Mouse and Zebrafish samples
Catalog #: 6110
$225.00

Product Details

Cat # +Size 6110-50
Size 50 μg
Antibody Target DNMT1
Alternate Name DNMT1
Host Mouse
Antibody Type Monoclonal
Isotype Mouse IgG1κ
Immunogen Synthetic peptide corresponding to amino acids 637-650 (EKDDREDKENAFKR) of human DNMT1
Accession # P26358
Gene ID 1786
Appearance Colorless liquid
Formulation 50 µg of antibody in 100 µl PBS containing 0.05% BSA and 0.05% sodium azide.
Purification Antigen affinity purified
Species Reactivity Human, mouse, zebrafish
Application Western blot, Immunohistochemistry, Immunoprecipitation, Chromatin Immunoprecipitation
Application & Usage Western blot: 2-4 µg/ml. IHC (parffin-embedded sections), ChIP, and IP: 1-2 µg/ml. However, the optimal conditions should be determined individually.
Handling The antibody solution should be gently mixed before use.
Storage Conditions -20 °C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting, and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, three families of mammalian DNA methyltransferase genes have been identified which include DNMT1, DNMT2, and DNMT3. DNMT1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. DNMT1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. DNMT1 also cooperates with Rb to repress transcription from promoters containing E2F-binding sites sμggesting a link between DNA methylation, histone deacetylase, and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells.


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