DNA Damage Quantification Colorimetric Kit (ab284577)
Key features and details
- Assay type: Cell-based (quantitative)
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Sample type: DNA
Overview
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Product name
DNA Damage Quantification Colorimetric Kit
See all DNA damage kits -
Detection method
Colorimetric/Fluorometric -
Sample type
DNA -
Assay type
Cell-based (quantitative) -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mammals -
Product overview
The DNA Damage Quantification Kit (ab284577) (K253) utilizes the ARP (Aldehyde Reactive Probe) reagent that reacts specifically with an aldehyde group which is the open ring form of the AP sites. After treating DNA containing AP sites with ARP reagents, AP sites are tagged with biotin residues, which can be quantified using avidin-biotin assay followed by a colorimetric detection. The kit provides the necessary reagents for convenient determination of abasic sites in purified DNA sample in 96-well plate format.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K253 DNA Damage Quantification Colorimetric Kit. K253-25 is the same size as the 25 test size of ab284577.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 25 tests 10X Wash Buffer III 1 x 30ml 96-Well Strip Plate 1 unit ARP Solution 1 x 125µl ARP-DNA Standard-0 1 x 0.6ml ARP-DNA Standard-40 1 x 0.6ml DNA Binding Solution 1 x 10ml Glycogen Solution 1 x 0.1ml HRP-Streptavidin 1 x 0.1ml TE Buffer I 1 x 30ml TMB Substrate I 1 x 10ml -
Research areas
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab284577 has not yet been referenced specifically in any publications.