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DNA Bisulfite Conversion Kit I

Spin Column based bisulfite DNA purification
Catalog #: K1478
SKU-Size Size Price Qty
K1478-50 50 Rxns
$225.00
K1478-200 200 Rxns
$475.00
More Sizes Get Quote

Product Details

Kit Summary Bisulfite modification of DNA and purification using spin columns
Detection Method Bisulfite modification of DNA and purification using spin columns
Applications An ideal tool for bisulfite modification of DNA and purification
Features & Benefits • Results ready in less than 2 h
• High recovery of DNA
• Many downstream applications such as Next generation Sequencing, PCR amplification, etc.
• Reliable and Ready-to-use
• High quality spin columns to recover bisulfite-treated DNA
Kit Components • *CT Conversion Reagent
• Dilution Buffer
• Dissolving Buffer
• Binding Buffer
• **Wash Buffer
• Desulphonation Buffer
• Elution Buffer
• Mini Column
• Collection Tube 2 ml
Storage Conditions -20ºC
Shipping Conditions RT
USAGE For Research Use Only! Not For Use in Humans.

Details

BioVision’s DNA Bisulfite Conversion Kit I is used for the bisulfite conversion of DNA that can be used to study the expression 5-methylcytosine. 5-methylcytosine is an epigenetic marker that plays an important role in differentiation, neurodegeneration, cancer etc. In this kit, DNA is treated with sodium bisulfite, which leads to the deamination of cytosine residues and conversion to uracil, while 5-methyl cytosine residues remain unaffected. DNA Bisulfite Conversion Kit I integrates the DNA denaturation and bisulfite conversion processes into a single step. The kit has been developed for the high recovery of DNA following DNA bisulfite conversion. This kit provides innovative in-column desulphonation technology that eliminates cumbersome DNA precipitation steps while providing researchers with consistent results every time. The kit is optimized to minimize template degradation, loss of DNA during treatment and clean-up, and to provide complete conversion of unmethylated cytosine residues. The DNA recovered from this kit is used for downstream applications such as endonuclease digestion, Next Generation Sequencing, microarrays, PCR amplification, etc.


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