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Recombinant DNA Binding Protein-7 (DBP-7)

Promotes annealing of complementary DNA strands
Catalog #: 7603
SKU-Size Size Price Qty
7603-20 20 μg
$180.00
7603-100 100 μg
$495.00
7603-1000 1 mg
$2,950.00
More Sizes Get Quote

Product Details

Alternate Name Chromodomain-helicase-DNA-binding protein 7 (CHD-7) (EC 3.6.4.12) (ATP-dependent helicase CHD7)
Gene Symbol CHD7
Gene ID 1454006
Accession # P39476
Source E. coli
Appearance Lyophilized protein
Physical Form Description Sterile filtered and lyophilized with no additives
Molecular Weight 9.44 kDa
Purity by SDS-PAGE ≥99%
Endotoxin Level <0.1 ng/μg
Reconstitution Instructions Reconstitute in ddH₂O to a concentration of 1.0 mg/ml. Aliquot and store at –20°C for future use. Repeated freeze/thaw cycles should be avoided.
Handling Centrifuge the vial prior to opening.
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not to be used in humans

Details

DNA Binding Protein-7 (DBP-7) is a N-terminal His-tagged recombinant protein and a member of the Sso7d family of small, abundant, non-specific DNA-binding proteins from the hyperthermophilic Archea Sulfolobus. The 7-kDa protein from Sulfolobus spp. consists of a five stranded, incomplete β-barrel capped at the opening by a C-terminal α-helix; they bind to the minor groove of a DNA duplex via the triple-stranded β-sheet. The topology of the Sulfolobus 7-kDa proteins was found to be similar to that of chromatin organization modifier (chromo) domains and eukaryotic SH3 domains, which are involved in protein–protein interactions. In vitro studies have shown that DBP-7 promotes the annealing of complementary DNA strands, induces negative supercoiling and chaperones the disassembly and renaturation of protein aggregates in an ATP hydrolysis-dependent manner.


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