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DLL4 (Human) ELISA Kit

A Sandwich ELISA kit for quantitative measurement of DLL4 in human serum, plasma, tissue lysates, and other biological fluids

WARNING: This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.
Catalog #: E5022
$750.00

Product Details

Cat # +Size E5022-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications Sandwich ELISA kit for quantitative measurement of DLL4 in serum, plasma, cell and tissue lysates, and other biological fluids
Features & Benefits ● Detection range: 78.125 – 5000 pg/ml
● Sensitivity: 46.875 pg/ml
● Assay Precision: Intra-Assay CV < 8% and Inter-Assay CV < 10%
● This Sandwich ELISA is highly sensitive and highly specific for the detection of DLL4 in human samples. There is no significant cross-reactivity or interference between DLL4 and analogues
● Recovery range: 85 - 105% for normal human serum and plasma samples
Kit Components ● Micro ELISA plate
● Wash Buffer (25X)
● Plate sealers
● Standard (Lyophilized) (5000 pg)
● Sample/Standard Dilution Buffer
● Biotin-labeled Antibody
● Antibody Dilution Buffer
● HRP-Streptavidin Conjugate (SABC)
● SABC Dilution Buffer
● TMB Substrate Solution
● Stop Solution
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Delta-like protein (DLL4) is a single-pass transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of proteins. DLL4 is one of the five ligands for Notch receptors. It plays a critical role in the Notch signaling pathway and promotes angiogenesis, blood homeostasis, and differentiation of blood vessels in either VEGF-dependent or VEGF-independent manner. In addition to angiogenesis, DLL4 also promotes migration and invasion of cancer cells. It is highly expressed in cancers such as colorectal, breast, and ovarian cancer. Several drugs, antibodies, antibody-drug conjugates are in development for DLL4 targeted therapies to treat cancer. BioVision’s DLL4 (Human) ELISA kit quantitatively measures DLL4 in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. HRP-Streptavidin is added to the plate, and post-incubation any unattached conjugates are washed off by Wash Buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of DLL4 in the sample or standard.


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