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DL-Serine Assay Kit (Fluorometric)

Market's first and only kit for enzymatic detection of Serine in biological samples
Catalog #: K743
$575.00

Product Details

Cat # +Size K743-100
Size 100 assays
Kit Summary • Detection method- Fluorescence (Ex/Em 535/587 nm)
• Application- Measurement of Serine in biological samples such as serum, plasma, CSF, etc.
Detection Method Fluorescence (Ex/Em 535/587 nm)
Species Reactivity Mammalian
Applications Estimation of Serine in various biological samples
Features & Benefits • Simple, rapid & convenient assay
• Limit of detection: 1 µM
• Very specific, does not interact with other standard and non-standard amino acids
Kit Components • Serine Assay Buffer
• Probe Solution
• Serine Racemase Enzyme Mix
• D-Serine Enzyme Mix
• Developer Enzyme Mix
• Sample Cleanup Mix
• D-Serine Standard
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Serine is one of the 20 naturally-occurring amino acids used by all organisms in the biosynthesis of proteins. Having a single chiral center, serine can exist as either of two stereoisomers (L-Serine and D-Serine). In addition to its role as a protein building block, L-Serine serves as a precursor to many vital biomolecules, including phosphatidylserine, sphingomyelin and the amino acids glycine and cysteine. The non-proteogenic isomer D-Serine is also synthesized from L-Serine in the mammalian brain. While most D-amino acids are only present in trace amounts in mammals, D-Serine is a vital neurotransmitter that acts as a co-agonist of synaptic N-methyl-D-aspartate (NMDA) type glutamate-gated ion channels. Impaired D-Serine metabolism may contribute to several neuropsychiatric disorders, including depression, schizophrenia, epilepsy and dementia. BioVision’s DL-Serine Assay Kit allows for quantification of both L- and D-Serine in biological fluids and tissues. The assay is based on the conversion of L-Serine to D-Serine, which is metabolized to an intermediate product that is subsequently oxidized and reacts with a probe to form a stable fluorophore (Ex/Em = 539/587 nm). Samples may be divided and assayed simultaneously for quantification of both D-Serine and total DL-Serine. The assay is not affected by physiological concentrations of other amino acids, is high-throughput adaptable and can detect less than 1 μM Serine


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Aimee L. Brauer, d-Serine Degradation by Proteus mirabilis Contributes to Fitness during Single-Species and Polymicrobial Catheter-Associated Urinary Tract Infection. mSphere, Feb 2019; 30814316.
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