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Dihydrotestosterone (DHT) ELISA Kit

A competitive ELISA kit for the quantitative measurement of Dihydrotestosterone in serum, plasma, tissue homogenates and other biological fluids.
Catalog #: E4604
$625.00

Product Details

Cat # +Size E4604-100
Size 100 assays
Kit Summary • Detection method- Absorbance (450 nm)
• Species reactivity- Universal
• Application- quantitative measurement of Dihydrotestosterone in serum, plasma, tissue homogenates and other biological fluids.
Detection Method Absorbance (450 nm)
Species Reactivity Universal
Applications This ELISA kit is used for quantitative measurement of Dihydrotestosterone inserum, plasma, tissue homogenates and other biological fluids.
Features & Benefits • Easy, convenient and time-saving method to measure the level of Dihydrotestosterone in serum, plasma, tissue homogenates, culture supernatants and other biological fluids.
• Detection Range: 39.063 - 2500 pg/ml
• Sensitivity: < 23.438 pg/ml
• Assay Precision: Intra-Assay: CV < 8%; Inter-Assay: CV < 10%
• No significant cross-reactivity or interference was observed.
Kit Components • Micro ELISA Plate
• Lyophilized Standard
• Sample / Standard dilution buffer
• Biotin- detection antibody (Concentrated)
• Antibody dilution buffer
• HRP-Streptavidin Conjugate (SABC)
• SABC dilution buffer
• TMB substrate
• Stop Solution
• Wash buffer (25X)
• Plate sealers
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Dihydrotestosterone (DHT), also known as androstanolone or stanolone, is an endogenous androgen sex steroid and hormone. DHT is biologically important for sexual differentiation of the male genitalia during embryogenesis, maturation of the penis and scrotum at puberty, growth of facial, body, and pubic hair, and development and maintenance of the prostate gland and seminal vesicles. The enzyme 5α-reductase catalyzes the formation of DHT from testosterone by mediates the reduction of the C4-5 double bond of testosterone. Relative to testosterone, DHT is considerably more potent as an agonist of the androgen receptor (AR). BioVision’s Dihydrotestosterone ELISA kit is a competitive ELISA assay for the quantitative measurement of Dihydrotestosterone in serum, plasma and cell culture supernatants. The density of color is proportional to the amount of Dihydrotestosterone captured from the samples.


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