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A thiol-reactive fluorescent probe
Catalog #: B2673
SKU-Size Size Price Qty
B2673-5 5 mg
B2673-25 25 mg
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Product Details

Alternate Name syn-(bromomethyl,methyl)bimane; NSC608550; Thiolyte BB; NSC-608550; 1,7-bis(bromomethyl)-2,6-dimethylpyrazolo[1,2-a]pyrazole-3,5-dione; dBBr
Appearance Crystalline solid
CAS # 68654-25-1
Molecular Formula C₁₀H₁₀Br₂N₂O₂
Molecular Weight 350.01
Purity ≥95%
Solubility DMSO
InChi InChI=1S/C10H10Br2N2O2/c1-5-7(3-11)13-8(4-12)6(2)10(16)14(13)9(5)15/h3-4H2,1-2H3
PubChem CID 125708
MDL Number MFCD00036952
Handling Do not take internally. Wear gloves and mask when handling the product! Avoid contact by all modes of exposure.
Storage Conditions -20ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Dibromobimane, a bifunctional thiol-reactive fluorescent reagent that is used as a cross-linking agent for cysteine mapping and studies on protein structure/conformation and cross-linking processes. It is a unique fluorogenic crosslinking reagent for proteins due to the fact that it is unlikely to fluoresce until both of its alkylating groups have reacted. The excitation and emission maxima for dibromobimane are 394 and 490 nm, respectively.

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