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Diazepam ELISA Kit

A Competitive ELISA kit for in vitro quantification of Diazepam concentrations in Tissue, Urine, Feed samples.
Catalog #: E4772

Product Details

Cat # +Size E4772-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Universal
Applications in vitro quantification of Diazepam concentrations in Tissue, Urine, Feed samples
Features & Benefits • Detection limit: Urine - 5 ppb, Tissue (chicken, beef, pork) - 5 ppb, Compound feed - 50 ppb, Condensed feed/ Premix feed - 100 ppb.
• Sensitivity: 0.3 ppb (ng/mL)
• Cross-reactivity: Diazepam - 100%, Nitrazepam - <10%, Oxazepam - <10%.
• Sample recovery rate: Tissue - 90%±20%
Kit Components • Reconstitution Buffer (2X)
• Plate Sealer
• Substrate Reagent B
• Antibody Working Solution
• Standard
• ELISA Microplate
• Substrate Reagent A
• HRP Conjugate
• Stop Solution
• Wash Buffer (20X)
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Diazepam is mainly used to treat anxiety, insomnia, panic attacks and symptoms of acute alcohol withdrawal. It is also used as a premedication for inducing sedation, anxiolysis, or amnesia before certain medical procedures (e.g., endoscopy). Diazepam is the drug of choice for treating benzodiazepine dependence with its long half-life allowing easier dose reduction. It is frequently employed in animal production for its excellent growth promotion properties. However, in humans, an overdose of diazepam leads to severe side effects. This has led to a prohibition of diazepam for the treatment of animals used for food production. BioVision’s Diazepam ELISA Kit is based on Competitive ELISA method. It can detect Diazepam in Tissue, Urine and Feed samples. The microtiter plate provided in the kit has been pre-coated with Diazepam. During the reaction, Diazepam in the samples or standard competes with coated Diazepam for anti- Diazepam antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each microtiter plate well, and TMB substrate is added for color development. There is a negative correlation between the OD value of samples and the concentration of Diazepam. The concentration of Diazepam in the samples can be calculated by comparing the OD of the samples to the standard curve.

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