Diamine Oxidase Activity Assay Kit (Fluorometric)

Catalog #: K496 | abID: ab241004

Product Details

abID ab241004
Cat # +Size K496-100
Size 100 assays
Kit Summary • Detection method: Fluorescence (Ex/Em = 535/587 nm)
• Application: Measurement of Total Diamine Oxidase (DAO) activity in various biological samples.
Detection Method Fluorescence (Ex/Em = 535/587 nm)
Species Reactivity Mammalian/Bacterial
Applications Measurement of Diamine Oxidase (DAO) in various biological samples.
Features & Benefits • Rapid & Convenient
• The assay can detect as little as 1 µU of DAO enzymatic activity.
• Includes DAO Enzyme as Positive Control
Kit Components • DAO Assay Buffer
• DAO Probe (in DMSO)
• DAO Substrate (Lyophilized)
• DAO Enzyme Mix (Lyophilized)
• H2O2 Standard (0.88 M)
• DAO Positive Control (Lyophilized)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Diamine Oxidase (DAO, E.C. 1.4.3.22) is an important regulator of polyamine levels in human physiology. DAO metabolizes the oxidative conversion of biogenic polyamines such as putrescine and cadaverine into aldehydes. Biogenic amines are known regulators of important biological pathways such as cell growth and signal transduction, placing DAO in an important regulatory position in maintaining cell viability. Putrescine itself is also an activator of c-jun and c-fos expression, further linking this diamine with potential roles in cancer development. DAO is notably involved in regulating growth and differentiation of intestinal mucosa, bone marrow, and other rapidly proliferating tissue. In addition to its role in tissue growth, DAO activity is important in metabolism. Loss of diamine oxidase activity can lead to histamine intolerance and histamine poisoning. BioVision’s Diamine Oxidase Activity Assay Kit provides a straightforward method to determine DAO activity of tissue lysates as well as recombinant enzymes, with a detection limit of less than 1 pmole/min of activity. In the assay, DAO converts the provided substrate, yielding an intermediate and H2¬¬O2. H2¬O2 is then utilized by the DAO Enzyme Mix to generate fluorescence (Ex/Em = 535/587 nm) from the DAO Probe.


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