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A RFP fluorophore mimic for imaging RNA in living cells
Catalog #: B3201
SKU-Size Size Price Qty
B3201-1 1 mg
B3201-set 1 set(5 x 1 mg)
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Product Details

Alternate Name 3,5-difluoro-4-hydroxybenzylidene imidazolinone-2-oxime
Peptide Sequence N/A
Appearance Orange solid
CAS # 1420815-34-4
Molecular Formula C₁₂H₉F₂N₃O₃
Molecular Weight 281.22
Purity ≥98% by HPLC
Solubility >25 mg/ml DMSO
InChi InChI=1S/C12H9F2N3O3/c1-17-10(5-15-20)16-9(12(17)19)4-6-2-7(13)11(18)8(14)3-6/h2-5,16,18H,1H3/b9-4-,10-5?
PubChem CID 146018929
Handling Do not take internally. Wear gloves and mask when handling the product! Avoid contact by all modes of exposure.
Storage Conditions -20ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Mimic of red fluorescent protein (RFP) fluorophore for imaging RNA in living cells. Upon binding to the Corn™ aptamer, DFHO is converted to a highly fluorescent state that can be detected at the emission wavelength of 545 nm. DFHO can also bind Broccoli™ derivative aptamers and turn on their fluorescence. DFHO is cell-permeable with negligible toxicity in living cells and can be used to label any genetically encoded Corn™ RNA tag. When bound to Corn™, DFHO has been found to be highly photostable compared to DFHBI (Cat. No. B2797), DFHBI-1T (B2799), and mVenus. The photostability of DFHO was demonstrated in the imaging and quantitative measurement of cellular Pol III promoter activity in living cells.

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