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A cell-permeable, non-toxic fluorogen
Catalog #: B2797
SKU-Size Size Price Qty
B2797-5 5 mg
B2797-25 25 mg
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Product Details

Alternate Name (5Z)-5-[(3,5-Difluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2,3-dimethyl- 4H-Imidazol-4-one; 3’5’-difluoro-4-hydroxybenzylidene
Peptide Sequence N/A
Appearance Yellow solid
CAS # 1241390-29-3
Molecular Formula C₁₂H₁₀F₂N₂O₂
Molecular Weight 252.22
Purity ≥95% by HPLC
Solubility ~20 mg/ml DMSO
InChi InChI=1S/C12H10F2N2O2/c1-6-15-10(12(18)16(6)2)5-7-3-8(13)11(17)9(14)4-7/h3-5,17H,1-2H3/b10-5-
PubChem CID 70808995
Handling Do not take internally. Wear gloves and mask when handling the product! Avoid contact by all modes of exposure.
Storage Conditions -20ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


DFHBI is a cell-permeable non-fluorescent small molecule derived from the GFP chromophore. Upon binding to the “Spinach™” aptamer, DFHBI is converted to a highly fluorescent state that can be detected at the emission wavelength of 501 nm. DFHBI displays negligible toxicity in living cells. DFHBI can be used to label any genetically encoded Spinach™ family of RNA tags that include Spinach™, Spinach2™, and Broccoli™. Live-cell imaging of tagged RNA can be performed using a standard fluorescence microscopy. Further, DFHBI can also selectively detect tagged RNA in total RNA gel electrophoresis.

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