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DFHBI-1T

A GFP fluorophore mimic for imaging RNA in living cells
Catalog #: B2799
SKU-Size Size Price Qty
B2799-1 1 mg
$95.00
B2799-5 5 mg
$375.00
More Sizes Get Quote

Product Details

Alternate Name (Z)-4-(3,5-difluoro-4-hydroxybenzylidene)-2-methyl-1-(2,2,2-trifluoroethyl)-1H-imidazol-5(4 H)-one; DFHBI1T
Peptide Sequence N/A
Appearance Yellow solid
CAS # 1539318-36-9
Molecular Formula C₁₃H₉F₅N₂O₂
Molecular Weight 320.21
Purity ≥98% by HPLC
Solubility >15 mg/ml Ethanol, >30 mg/ml DMSO
SMILES CC1=NC(=CC2=CC(=C(C(=C2)F)O)F)C(=O)N1CC(F)(F)F
InChi InChI=1S/C13H9F5N2O2/c1-6-19-10(12(22)20(6)5-13(16,17)18)4-7-2-8(14)11(21)9(15)3-7/h2-4,21H,5H2,1H3/b10-4-
InChi Key AWYCLBWNRONMQC-WMZJFQQLSA-N
PubChem CID 101889712
MDL Number MFCD31628972
Handling Do not take internally. Wear gloves and mask when handling the product! Avoid contact by all modes of exposure.
Storage Conditions -20 °C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

DFHBI-1T is a mimic of green fluorescent protein (GFP) fluorophore for imaging RNA in living cells. Fluorescence is activated by binding to Spinach2 or Broccoli aptamers. Displays peak excitation maxima of 482 nm and peak fluorescence emission of 505 nm when bound to Spinach2, enabling imaging with GFP filter cubes. DFHBI-1T has been found to have lower background fluorescence than DFHBI (Cat. No. B2797) and exhibits an overall increase in brightness in living cells. DFHBI-1T is cell-permeable with negligible toxicity in living cells and can be used to label any genetically encoded Spinach™, Spinch2™, and Broccoli™ RNA tags.


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