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Product Details
| Cat # +Size | E5131-100 |
|---|---|
| Size | 96 assays |
| Detection Method | Absorbance is measured at 450 nm |
| Species Reactivity | Human |
| Applications | The ELISA kit is used for the quantitative detection of Desmoglein-2 in serum, plasma, tissue homogenates and other biological fluids |
| Features & Benefits | ● Recovery Rate: 87-102% for serum, 87-101% for EDTA plasma and 91-104% for heparin plasma ● Assay Precision; Intra-Assay CV < 8% and Inter-Assay < 10% ● Sensitivity: 0.094 ng/ml ● Highly sensitive and specific ● Detection range: 0.156-10 ng/ml |
| Kit Components | ● ELISA Microplate ● Wash Buffer (25X) ● Plate Sealers ● Lyophilized Standard (10 ng) ● Sample/Standard Dilution Buffer ● Biotin-labeled Antibody (Concentrated) ● Antibody Dilution Buffer ● HRP-Streptavidin Conjugate (SABC) ● SABC Dilution Buffer ● TMB Substrate ● Stop Solution |
| Storage Conditions | 4ºC |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
Desmoglein 2 is a member of the desmosomal cadherin family that comprise the desmogleins and desmocollins. Desmogleins and desmocollins connect extracellularly via homophilic and heterophilic interactions. Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. Desmoglein-2 is a 122.2 kDa protein composed of 1118 amino acids. It is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate cells. Desmoglein-2 is highly expressed in epithelial cells and cardiomyocytes. Desmoglein-2 is localized to desmosome structures at regions of cell-cell contact and functions to structurally adhere adjacent cells together. Mutations in desmoglein-2 have been associated with arrhythmogenic right ventricular cardiomyopathy and familial dilated cardiomyopathy. Increased Desmoglein 2 expression is associated with tumor progression in hepatocellular carcinoma and functions as a promising biomarker for unfavorable prognosis of hepatocellular carcinoma. BioVision’s Desmoglein-2 (Human) ELISA Kit is used for the quantitative detection of Desmoglein-2 in serum, plasma, tissue homogenates and other biological fluids. It is based on the principle of sandwich ELISA. The capture antibody is pre-coated on 96-well plates. The standards, test samples and biotin conjugated detection antibody are added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin is added and unbound conjugates are washed away with wash buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of Desmoglein-2 in the sample.
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