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Desmoglein-1 (Human) ELISA Kit

A highly sensitive sandwich ELISA kit for the quantitative measurement of Desmoglein-1

WARNING: This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.
Catalog #: E5129
$750.00

Product Details

Cat # +Size E5129-100
Size 96 assays
Detection Method Absorbance is measured at 450 nm
Species Reactivity Human
Applications The ELISA kit is used for the quantitative detection of Desmoglein-1 in serum, plasma, tissue homogenates and other biological fluids
Features & Benefits ● Sensitivity: 0.188 ng/ml
● Detection range: 0.313-20 ng/ml
● Recovery Rate: 86-104 % for serum, 88-105% for EDTA plasma and 87-100% for heparin plasma
● Highly sensitive and specific
● Assay Precision; Intra-Assay CV < 8% and Inter-Assay < 10%
Kit Components ● ELISA Microplate
● Wash Buffer (25X)
● Plate Sealers
● Lyophilized Standard (20 ng)
● Sample/Standard Dilution Buffer
● Biotin-labeled Antibody (Concentrated)
● Antibody Dilution Buffer
● HRP-Streptavidin Conjugate (SABC)
● SABC Dilution Buffer
● TMB Substrate
● Stop Solution
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Desmoglein 1 is a member of the desmosomal cadherin family that comprise the desmogleins and desmocollins. Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. Desmoglein-1 is the target antigen in majority of the cases linked to IgG/IgA pemphigus, which is an autoimmune IgG/IgA antibody mediated response. It is an essential intercellular adhesion molecule that is altered in various human cutaneous disorders. Desmoglein-1 is a target of Staphylococcus Exotoxins A and B which contribute to the pathoaetiology of Staph Scalded Skin Syndrome. Deficiency of the Desmoglein-1 protein has been found to be associated with increased expression of multiple genes encoding allergy-related cytokines. It is haploinsufficient and a mutation in the gene can cause the autosomal dominant mutation striate palmoplantar keratoderma. BioVision’s Desmoglein-1 (Human) ELISA Kit is used for the quantitative detection of Desmoglein-1 in serum, plasma, tissue homogenates and other biological fluids. It is based on the principle of sandwich ELISA. The capture antibody is pre-coated on 96-well plates. The standards, test samples and biotin conjugated detection antibody are added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin is added and unbound conjugates are washed away with wash buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of Desmoglein-1 in the sample.


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