Deproteinizing Sample Preparation Kit - PCA (ab284939)
Key features and details
- Sample type: Cell Lysate, Tissue Homogenate, Urine
Overview
-
Product name
Deproteinizing Sample Preparation Kit - PCA
See all Deproteinizing sample kits -
Sample type
Urine, Cell Lysate, Tissue Homogenate -
Assay duration
Multiple steps standard assay -
Product overview
The Deproteinizing Sample Preparation Kit - PCA (ab284939) (K808) utilizes a PCA (perchloric acid) precipitation method, providing a unique tool to prepare samples for the analysis of a variety of small molecules. Using the kit, proteins are precipitated, excess PCA is removed, samples are neutralized. Samples prepared using this kit can be directly used in a wide variety of bioassays.
The method is easy, convenient, and can be used for the preparation of a large number of samples.
Properties
-
Storage instructions
Store at room temperature. Please refer to protocols. -
Components 200 tests Neutralization Buffer I 1 x 4ml Perchloric Acid Solution 1 x 20ml -
Research areas
Images
-
Different amounts of PCA were used to deproteinize 400 µL of serum following the kit protocol. Protein concentration remaining in solution OD280 (♦) and sample pH (•) was measured. Samples (neat serum ; serum filtered through a 10kD MW cutoff ; and PCA treated ) were diluted 20 times for convenience of measuring OD at 280 nm. A separate aliquot of the same PCA treated samples was mixed 1:1 with a pH 7.75 assay buffer and the pH of the mixture determined. The figure shows that sample/PCA ratio errors up to 20% can be tolerated with the resulting assay pH holding within approximately 0.5 pH units of the target assay pH. Using a 10 kDa MW cutoff filter removes approximately 98% of protein and PCA precipitation at a PCA/sample ratio of 1:4 removes approximately 95% of protein present.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab284939 has not yet been referenced specifically in any publications.