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DEPC-Treated Water

RNase- and DNase-free water
Catalog #: M1500
SKU-Size Size Price Qty
M1500-500 500 ml
$95.00
M1500-1000 1000 ml
$145.00
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Product Details

Highlights • Ready-to-use Solution
• For RNA experiments
• RNase and DNase Free
• Molecular Biology Grade
Storage Conditions RT
Shipping Conditions RT
USAGE For Research Use Only! Not For Use in Humans.

Details

DEPC is a non-specific inhibitor of Ribonucleases (RNases). To reduce degradation of RNA in experiments, water is treated with DEPC. A concentration of 0.1 % DEPC is used to reduce RNase contamination in laboratory procedures. DEPC modifies enzymes containing -NH, -SH, or -OH groups in their active sites. DEPC particularly reacts with Cys, His, Lys, Ser and Tyr residues of proteins. DEPC-Treated Water is prepared by adding DEPC to water and autoclaving the solution. Autoclaving inactivates DEPC by causing hydrolysis of DEPC and release of by-products ethanol and carbon-dioxide. DEPC has a half-life of approximately 30 min in water. DEPC-Treated Water (0.1%) can be used with buffers such as PBS and MOPS but cannot be used with the buffers Tris and HEPES. Tris and HEPES make DEPC unavailable to inactivate RNase. DEPC-treated water should not be added to aqueous solutions of ammonia as it will result in the formation of urethane, a possible carcinogen.


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