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Deoxyribonuclease-2-alpha (DNase II alpha) (Human) ELISA Kit

A Sandwich ELISA kit for the quantitative measurement of DNase II alpha in serum, plasma, tissue homogenates and other biological fluids.
Catalog #: E4820
$725.00

Product Details

Cat # +Size E4820-100
Size 96 assay
Detection Method Sandwich ELISA, Absorbance (450 nm)
Species Reactivity Human
Applications A Sandwich ELISA kit for the quantitative measurement of DNase II alpha in serum, plasma, tissue homogenates and other biological fluids.
Features & Benefits • Sensitivity: 46.88 pg/ml
• Assay Precision: Intra-Assay: CV < 8%; Inter-Assay: CV < 10%
• This assay has high sensitivity and excellent specificity for detection of DNase II. No significant crossreactivity or interference between DNase II and analogues was observed.
• Detection Range: 78.13-5000 pg/ml
Kit Components • Micro ELISA Plate
• Lyophilized Standard (5000 pg)
• Sample / Standard dilution buffer
• Biotin- labeled antibody
• Antibody dilution buffer
• HRP-Streptavidin Conjugate (SABC)
• SABC dilution buffer
• TMB substrate
• Stop Solution
• Wash buffer (25X)
• Plate sealers
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Deoxyribonuclease-2-alpha hydrolyzes DNA under acidic conditions with a preference for double-stranded DNA. It plays a major role in the degradation of nuclear DNA in cellular apoptosis during development. DNase II alpha is necessary for proper fetal development and for definitive erythropoiesis in fetal liver, where it degrades nuclear DNA expelled from erythroid precursor cells. BioVision's Deoxyribonuclease-2-alpha (DNase II alpha) (Human) ELISA Kit is based on the Sandwich ELISA Principle. A 96-well plate was coated with a capture antibody and the biotin conjugated antibody was used as detection antibody. The standards, test samples, and biotin conjugated detection antibody were added to the wells subsequently and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding the acidic stop solution. The density of color is proportional to the amount of DNase II alpha captured from the samples.


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