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Deoxyribonuclease-1 (DNase1) (Human) ELISA Kit

A Sandwich ELISA kit for the quantitative measurement of DNase1 in human serum, plasma, culture supernatants and other biological fluids.
Catalog #: E4500

In stock

$695.00

Product Details

Cat # +Size E4500-100
Size 100 assays
Kit Summary • Detection method- Absorbance (450 nm)
• Species reactivity- Human
• Application- quantitative measurement of DNase1 in human serum, plasma, tissue homogenates and other biological fluids.
Detection Method Human
Species Reactivity Human
Applications Quantitative measurement of DNase1 in human serum, plasma, tissue homogenates and other biological fluids.
Features & Benefits • Easy, convenient and time-saving method to measure the level of DNase1 in human serum, plasma, tissue homogenates, culture supernatants and other biological fluids.
• Detection Range: 0.156 - 10 ng/ml
• Sensitivity: < 0.094 ng/ml
• Assay Precision: Intra-Assay: CV < 8%; Inter-Assay: CV < 10%
• This assay has high sensitivity and excellent specificity for detection of DNase1. No significant cross-reactivity or interference between DNase1 and analogues was observed.
Kit Components • Micro ELISA Plate
• Lyophilized Standard
• Sample / Standard dilution buffer
• Biotin- detection antibody (Concentrated)
• Antibody dilution buffer
• HRP-Streptavidin Conjugate (SABC)
• SABC dilution buffer
• TMB substrate
• Stop Solution
• Wash buffer (25X)
• Plate sealers
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

DNase I is a nuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, yielding 5'-phosphate-terminated polynucleotides with a free hydroxyl group on position 3', on average producing tetranucleotides. It acts on single-stranded DNA, double-stranded DNA, and chromatin. In addition to its role as a waste-management endonuclease, it has been suggested to be one of the deoxyribonucleases responsible for DNA fragmentation during apoptosis. BioVision’s DNase1 ELISA kit is a sandwich ELISA assay for the quantitative measurement of DNase1 in human serum, plasma and cell culture supernatants. The density of color is proportional to the amount of DNase1 captured from the samples.


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