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DECTIN-1 (Human) ELISA Kit

A Sandwich ELISA kit for quantitative measurement of DECTIN-1 in human serum, plasma, tissue lysates, and other biological fluids

WARNING: This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.
Catalog #: E5023
$750.00

Product Details

Cat # +Size E5023-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications Sandwich ELISA kit for quantitative measurement of DECTIN-1 in serum, plasma, cell and tissue lysates, and other biological fluids
Features & Benefits ● This Sandwich ELISA is highly sensitive and highly specific for the detection of DECTIN-1 in human samples. There is no significant cross-reactivity or interference between DECTIN-1 and analogues
● Assay Precision: Intra-Assay CV < 8% and Inter-Assay CV < 10%
● Sensitivity: 18.75 pg/ml
● Recovery range: 85 - 105% for normal human serum and plasma samples
● Detection range: 31.25 – 2000 pg/ml
Kit Components ● Micro ELISA plate
● Wash Buffer (25X)
● Plate sealers
● Standard (Lyophilized) (5000 pg)
● Sample/Standard Dilution Buffer
● Biotin-labeled Antibody
● Antibody Dilution Buffer
● HRP-Streptavidin Conjugate (SABC)
● SABC Dilution Buffer
● TMB Substrate Solution
● Stop Solution
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

DECTIN-1 is a 28 kDa type II transmembrane protein that belongs to the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. It is expressed on macrophages, monocytes, neutrophils, and T-cells. DECTIN-1 recognizes the β-1,3-linked and β-1,6-linked glucans on the cell wall of fungi and bacteria and mediates an innate immune response against these fungal and bacterial pathogens. It triggers the expression of multiple inflammatory cytokines and chemokines such as TNF, IL-2, IL-6 and prevents fungal infection. Other functions of DECTIN-1 include respiratory burst, maturation of dendritic cells, and phagocytosis of the ligand. Therefore, DECTIN-1 could be considered a promising target for the treatment of infectious diseases. BioVision’s DECTIN-1 (Human) ELISA kit quantitatively measures DECTIN-1 in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. HRP-Streptavidin is added to the plate, and post-incubation any unattached conjugates are washed off by Wash Buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of DECTIN-1 in the sample or standard.


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