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D-Sorbitol Assay Kit II (Colorimetric)

A quick and easy way to determine accurately the amount of sorbitol in a variety of samples such as foods, fruits, beverages, pharmaceuticals, cosmetics and other biological samples.
Catalog #: K2119
$525.00

Product Details

Cat # +Size K2119-100
Size 100 assays
Kit Summary BioVision’s D-Sorbitol Assay Kit II provides a quick and easy way to determine accurately the amount of sorbitol in a variety of samples such as foods, fruits, beverages, pharmaceuticals, cosmetics and other biological samples.
Detection Method Absorbance at 460 nm
Applications Measurement of sorbitol in foodstuffs, beverages, plant extracts, etc.
Features & Benefits The assay is simple, rapid and can detect a minimum of 0.1 nmoles/well of sorbitol (~5 μM in a 20 μl sample volume).
Kit Components ● Sorbitol Assay Buffer
● Sorbitol Probe
● Sorbitol Enzyme Mix
● Sorbitol Standard (100 mM)
Storage Conditions -20ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Sorbitol (D-glucitol) is a 6-carbon sugar alcohol naturally produced in plants, obtained by the reduction of the aldehyde moiety of glucose. It is one of the main photosynthetic end products and serves as a storage and transport sugar in most plant families, and plays an important role in osmotic adjustment in cell cytoplasm under various abiotic stresses such as salinity, chilling, and drought. It is found in abundance in fruits such as apples, berries and stone fruits (e.g. peaches, apricots and plums). Sorbitol is commonly used as an artificial sweetener, as a laxative and in cosmetics as a humectant and thickening agent. It can be produced in humans in small amounts by the reduction of glucose by aldose reductase. Due to its poor ability to diffuse across the cell membrane, sorbitol can become trapped in cells and is believed to be one of the causes of neuropathic damage (due to osmotic effects) in diabetes. Interestingly, sorbitol can be used as a screen for the O154:H7 strain of E. coli, since this strain is one of the few strains which cannot metabolize sorbitol. BioVision’s D-Sorbitol Assay Kit II provides a quick and easy way to determine accurately the amount of sorbitol in a variety of samples such as foods, fruits, beverages, pharmaceuticals, cosmetics and other biological samples. In this Assay, sorbitol is oxidized to fructose, with the proportional reduction of a colorimetric probe that has an absorption maximum at 460 nm. The assay is simple, rapid and can detect a minimum of 0.1 nmoles/well of sorbitol (~5 μM in a 20 μl sample volume).


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