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D-Glucose Isomerase, Recombinant

Involved in D-xylose catabolism
Catalog #: P1307
SKU-Size Size Price Qty
P1307-100 100 mU
P1307-500 500 mU
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Product Details

Alternate Name D-Xylose Isomerase, fructose-isomerase, D-xylose aldose-ketose-isomerase, D-xylose ketoisomerase, D-xylose ketol-isomerase
Gene Symbol xylA
Accession # P24300
Source E.coli
Appearance Ammonium sulfate suspension
Physical Form Description In 50 mM Tris-HCl buffer (pH 7.0) containing 30% (NH4)2SO4 and 1 mM MgCl2
Molecular Weight 45.4 kDa
Purity by SDS-PAGE ≥95%
Biological Activity > 5 mU/mg. The activity of D-Glucose isomerase is determined by using the D-Glucose Isomerase Activity Assay Kit (Cat. No. K177).
Reconstitution Instructions Spin at >300 x g for 10 minutes and remove the supernatant. Dissolve the pellets in PBS or appropriate buffer.
Handling Centrifuge the vial prior to opening.
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not to be used in humans


Glucose Isomerase catalyzes the reversible isomerization of glucose to fructose and that of xylose to xylulose. The enzyme is widely used in the industrial production of high-fructose corn syrup (HFCS) as well as in bioethanol fermentation from hemicellulosic hydrolysates.Recombinant Glucose Isomerase protein (S. rubiginosus), fused to His-tag at N-terminus, was expressed in E.coli and purified using propriety chromatography techniques.

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