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D-Dimer (Human) ELISA Kit

A Sandwich ELISA kit for quantitative measurement of D-Dimer in human serum, plasma, tissue lysates, and other biological fluids

WARNING: This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California to cause cancer. For more information go to
Catalog #: E5020

Product Details

Cat # +Size E5020-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications Sandwich ELISA kit for quantitative measurement of D-Dimer in serum, plasma, cell and tissue lysates, and other biological fluids
Features & Benefits ● Detection range: 78.125 – 5000 pg/ml
● Sensitivity: 46.875 pg/ml
● This Sandwich ELISA is highly sensitive and highly specific for the detection of D-Dimer in human samples. There is no significant cross-reactivity or interference between D-Dimer and analogues
● Assay Precision: Intra-Assay CV < 8% and Inter-Assay CV < 10%
● Recovery range: 85 - 105% for normal human serum and plasma samples
Kit Components ● Micro ELISA plate
● Wash Buffer (25X)
● Plate sealers
● Standard (Lyophilized) (5000 pg)
● Sample/Standard Dilution Buffer
● Biotin-labeled Antibody
● Antibody Dilution Buffer
● HRP-Streptavidin Conjugate (SABC)
● SABC Dilution Buffer
● TMB Substrate Solution
● Stop Solution
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


D-Dimer (D2D) is a small protein fragment present in the blood when the blood clot undergoes degradation by fibrinolysis. As a result of tissue or blood vessel injury, the human body initiates a process called hemostasis to create a blood clot to halt bleeding. During the clot formation, the D subunits form a bond with two platelets. When the injured tissue/blood vessel begins to heal, the blood clot breaks down into smaller fibrin degradation products. One of these fibrin degradation products is called a D-Dimer. D-Dimer currently serves as an efficient biomarker to detect blood clotting disorders such as Deep Vein Thrombosis (DVT), Pulmonary embolism (PE), Disseminated intravascular coagulation (DIC), and stroke. BioVision’s D-Dimer (Human) ELISA kit quantitatively measures D-Dimer in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. HRP-Streptavidin is added to the plate, and post-incubation any unattached conjugates are washed off by Wash Buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of D-Dimer in the sample or standard.

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