Product Details
| abID | ab18738 |
|---|---|
| Antibody Target | Cytochrome c |
| Alternate Name | CYCS, CYC |
| Host | Rabbit |
| Antibody Type | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | Synthetic peptide corresponding to residues surrounding amino acid 70 of rat cytochrome c |
| Accession # | NP_036972 |
| Gene ID | 25310 |
| Appearance | Colorless liquid |
| Formulation | In phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.03% proclin |
| Purification | Affinity purified |
| Species Reactivity | Human, Mouse, Rat, Bovine, Chicken, Dog |
| Application | Western blot, Immunoprecipitation |
| Application & Usage | Western blotting (0.5-4 µg/ml) and immunoprecipitation (10-20 µg/ml). The cytochrome c antibody detects the 12.6 kDa cytochrome c from human, mouse, and rat samples. Jurkat cell lysate, NIH3T3 cell lysate, and rat kidney tissue lysate can be used as posit |
| Handling | The antibody solution should be gently mixed before use. |
| Storage Conditions | -20 °C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
Cytochrome c (m.w. 12,500) is an electron transport protein from mitochondria. It is released from mitochondria to cytoplasm during the early stages of apoptosis, prior to caspase activation, DNA fragmentation, and loss of membrane potential. The cytoplasmic cytochrome c is associated with Apaf-1 and caspase-9 to activate caspase-3 and other caspases
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Lan L et al (2008) J. Immunol. 181: 4926 - 4935.
Zhao J et al (2008) Cancer Res. 68: 4133 - 4141.
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