Cytochrome c Antibody

Catalog #: 3352 | abID:

Product Details

Antibody Target Cytochrome c
Host Rabbit
Antibody Type Polyclonal
Isotype Rabbit IgG
Immunogen Rat cytochrome c
Accession # NP_036971
Gene ID 6978725
Appearance Colorless liquid
Concentration 0.2 mg/ml
Formulation 100 µg (0.2 mg/ml) affinity purified rabbit anti-Cytochrome c polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
Purification Affinity purified
Species Reactivity rat
Application Western blot
Application & Usage Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Rat kidney lysate (Cat.# 2702-100) can be used as a positive control. The cytochrome c antibody detects the 12 kDa cytochrome c from rat samples.
Handling The antibody solution should be gently mixed before use.
Storage Conditions -20 °C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Cytochrome c (m.w. 12,500) is an electron transport protein from mitochondria. It is released from mitochondria to cytoplasm during the early stages of apoptosis, prior to caspase activation, DNA fragmentation, and loss of membrane potential. The cytoplasmic cytochrome c is associated with Apaf-1 and caspase-9 to activate caspase-3 and other caspases.

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Stefan Broselid et al., G Protein–Coupled Estrogen Receptor Is Apoptotic and Correlates with Increased Distant Disease-Free Survival of Estrogen Receptor–Positive Breast Cancer Patients. Clin. Cancer Res., Apr 2013; 19: 1681 - 1692.
Tetsuya Kotani et al., Human G-proteins, ObgH1 and Mtg1, associate with the large mitochondrial ribosome subunit and are involved in translation and assembly of respiratory complexes. Nucleic Acids Res., Apr 2013; 41: 3713 - 3722.
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