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Cytidine Deaminase Activity Assay Kit (Fluorometric)

Rapid & simple assay to measures total Cytidine deaminase activity
Catalog #: K451

In stock

$365.00

Product Details

Cat # +Size K451-100
Size 100 assays
Kit Summary • Detection method- Fluorescence (Ex/Em = 410/470 nm)
• Application- Detection of Cytidine Deaminase activity
Detection Method Fluorescence (Ex/Em = 410/470nm)
Species Reactivity Eukaryotes
Applications • Measurement of CDA activity in various tissues/cells
• Evaluation of CDA activity for comparison of activity rates in normal versus tumor tissues
Features & Benefits • Rapid, and convenient
• The kit measures total Activity of Cytidine Deaminase with limit of quantification 1 µU recombinant Cytidine Deaminase. Range of the assay: 10-100 µU.
Kit Components • CDA Assay Buffer
• CDA Substrate
• Cytidine Deaminase (CDA)
• Developer A
• Ammonium Chloride Standard
• Microplate Sealing Film
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Cytidine Deaminase (3.5.4.5) is a homotetramer enzyme that catalyzes the irreversible deamination of cytidine to produce uridine, thus maintaining the intracellular pyrimidine pool of uridine. Cytidine as well as adenosine, thymidine, guanosine and uridine are one of the five standard nucleosides with which nucleic acids are composed. Nucleosides accumulate or are recycled from salvage pathways within the cell and play an important role in DNA and RNA synthesis and subsequent cell division. Tumorigenesis is intricately linked to metabolism of macromolecular precursors, specifically, the building blocks of DNA and RNA. Thus tumor cells have greater concentrations of uridine and cytidine analogues (402 µM +/- 252) compared to normal cells (83 µM +/- 133), to support the increased demand for nucleotides in mitosis in actively dividing cells. BioVision’s Cytidine Deaminase Activity Assay Kit uses CDA to convert cytidine to the uridine and NH3, as intermediates. The intermediate products then react with a proprietary Reaction Mix to generate a stable fluorophore that can be detected fluorometrically (Ex/Em= 410/470 nm). The kit is suitable for measuring CDA activity as low as 1 µUnit (1 pmole/min/well) in biological samples


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