Call 408.493.1800 | Fax 408.493.1801 | Toll Free 800.891.9699 (US Only) | Email: [email protected]

Cystathionine β-Synthase Inhibitor Screening Kit (Fluorometric)

Catalog #: K695

Product Details

Cat # +Size K695-100
Size 100 assays
Detection Method Fluorescence (Ex/Em 368/460 nm)
Applications Screening and characterization of inhibitors of Cystathionine β Synthase (CβS)
Features & Benefits • Simple and High throughput adaptable
• Fluorometric Detection
• Screen within 60 min
Kit Components • CβS Assay Buffer
• CβS Probe (in DMSO)
• CβS Substrate
• Cofactor 1
• Cofactor 2
• Reducing Agent
• CβS
• AOAA (100 mM in DMSO)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Cystathionine β Synthase (EC, CβS) is a Pyridoxal 5-Phosphate-dependent enzyme that uses cysteine and homocysteine as substrates to catalyze the formation of H2S and cystathionine. CβS is well-known for its role in human sulfur metabolism. The overexpression of CβS has been implicated in Down Syndrome, and also results in homocystinuria; or low levels of homocysteine in blood. Homocystinuria leads to a mutli-systemic disorder of the connective tissue, muscles, and central nervous system (CNS). For that reason, pharmacological inhibitors of human CβS present attractive therapeutic potential in order to restore baseline CβS activity and blood homocysteine levels. It has been shown that aminooxyacetic acid (AOAA) is an irreversible inhibitor that targets the PLP-binding site of CβS. That interaction inhibits the transaminase reaction that would yield cystathionine and H2S in wildtype conditions. BioVision’s Cystathionine β Synthase Inhibitor Screening Kit is the first available kit that enables customers to screen inhibitors of CβS and quantify their therapeutic potential. The kit has a simple, easy-to-follow protocol and is high-throughput adaptable. In this kit, the product hydrogen sulfide reacts with the non-fluorescent azido-functional group to yield a fluorescent amino group (Ex/Em = 368/460 nm). In the presence of AOAA, CβS activity is inhibited, which subsequently abolishes the production of H2S and thus the fluorescent signal is reduced.

Why buy BioVision Products?
Global Presence
Technical Support
BioVision aims to provide our customers innovative tools for accelerating drug discovery and biological research. BioVision offers >8,000 products including the most comprehensive array of assay kits for key targets in Metabolic pathways.
BioVision is committed to providing the highest quality products at a competitive price.
We have a broad network of global distributors who are ready to address your research needs and ensure fast delivery.
Our highly trained Technical Support team provides comprehensive product support and is dedicated to resolving your issues quickly and efficiently.