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Cystathionine β-Synthase Inhibitor Screening Kit (Fluorometric)

Catalog #: K695
$525.00

Product Details

Cat # +Size K695-100
Size 100 assays
Detection Method Fluorescence (Ex/Em 368/460 nm)
Applications Screening and characterization of inhibitors of Cystathionine β Synthase (CβS)
Features & Benefits • Simple and High throughput adaptable
• Fluorometric Detection
• Screen within 60 min
Kit Components • CβS Assay Buffer
• CβS Probe (in DMSO)
• CβS Substrate
• Cofactor 1
• Cofactor 2
• Reducing Agent
• CβS
• AOAA (100 mM in DMSO)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Cystathionine β Synthase (EC 4.2.1.22, CβS) is a Pyridoxal 5-Phosphate-dependent enzyme that uses cysteine and homocysteine as substrates to catalyze the formation of H2S and cystathionine. CβS is well-known for its role in human sulfur metabolism. The overexpression of CβS has been implicated in Down Syndrome, and also results in homocystinuria; or low levels of homocysteine in blood. Homocystinuria leads to a mutli-systemic disorder of the connective tissue, muscles, and central nervous system (CNS). For that reason, pharmacological inhibitors of human CβS present attractive therapeutic potential in order to restore baseline CβS activity and blood homocysteine levels. It has been shown that aminooxyacetic acid (AOAA) is an irreversible inhibitor that targets the PLP-binding site of CβS. That interaction inhibits the transaminase reaction that would yield cystathionine and H2S in wildtype conditions. BioVision’s Cystathionine β Synthase Inhibitor Screening Kit is the first available kit that enables customers to screen inhibitors of CβS and quantify their therapeutic potential. The kit has a simple, easy-to-follow protocol and is high-throughput adaptable. In this kit, the product hydrogen sulfide reacts with the non-fluorescent azido-functional group to yield a fluorescent amino group (Ex/Em = 368/460 nm). In the presence of AOAA, CβS activity is inhibited, which subsequently abolishes the production of H2S and thus the fluorescent signal is reduced.


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