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CTGF (Human) ELISA Kit

A Sandwich ELISA kit for the quantitative measurement of human CTGF in Serum, plasma and other biological fluids.
Catalog #: E4837
$725.00

Product Details

Cat # +Size E4837-100
Size 96 assays
Kit Summary CCN2, HCS24, IGFBP8, Connective Tissue Growth Factor
Detection Method Sandwich ELISA, Absorbance (450 nm)
Species Reactivity Human
Applications • A Sandwich ELISA kit for the quantitative measurement of human CTGF in Serum, plasma and other biological fluids.
Features & Benefits • Sensitivity: 37.50 pg/mL
• Precision: Coefficient of variation is < 10%.
• Detection Range: 62.50-4000 pg/mL
• Specificity: This kit recognizes Human CTGF in samples. No Significant cross-reactivity or interference between Human CTGF and analogues was observed.
Kit Components • Micro ELISA Plate
• Reference Standard
• Biotinylated Detection Ab (100x)
• HRP Conjugate (100x)
• Reference Standard & Sample Diluent
• Biotinylated Detection Antibody Diluent
• HRP Conjugate Diluent
• Wash Buffer (25X)
• Substrate Reagent
• Stop Solution
• Plate Sealer
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

CTGF is a major connective tissue mitoattractant secreted by vascular endothelial cells. It promotes proliferation and differentiation of chondrocytes. It also mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells and enhances fibroblast growth factor-induced DNA synthesis. BioVision’s CTGF (Human) ELISA Kit is based on the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CTGF. Standards or samples are added to the micro ELISA plate wells that bind to the specific antibody. Then a biotinylated detection antibody specific for Human CTGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. The wells are washed, a TMB substrate solution is added to the wells and blue color develops in proportion to the amount of CTGF bound. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Human CTGF. The concentration of Human CTGF in the samples can be calculated by comparing the OD of the samples to the standard curve.


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