Crystal Violet Cell Cytotoxicity Assay Kit

Catalog #: K329 | abID: ab232855

Product Details

abID ab232855
Cat # +Size K329-1000
Size 1000 assays
Kit Summary
• Detection method- Absorbance (570 nm)
• Sample type- Cell culture (adherent and non adherent)
• Species reactivity- Mammalian
• Applications- This assay provides a convenient and non-radioactive alternative to carry out cytotoxicity assays. This assay relies on the ability of crystal violet to bind ribose molecules (i.e. DNA) in viable cells.
Detection Method Detection method- Absorbance (570 nm)
Species Reactivity Mammalian
Applications This assay provides a convenient and non-radioactive alternative to carry out cytotoxicity assays.This assay relies on the ability of crystal violet to bind ribose molecules (i.e. DNA) in viable cells.
Features & Benefits
• Simple procedure; takes less than 2 hours
• Fast, sensitive and convenient
Kit Components Crystal Violet Staining Solution
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Crystal violet (CV) cell cytotoxicity assay is one of the common methods used to detect cell viability or drug cytotoxicity. CV is a triarylmethane dye that can bind to ribose type molecules such as DNA in nuclei. Normally, dead adherent cells will detach from cell culture plates and will be removed from viable cell propulation during washing steps. CV staining can be used to quantify the total DNA of the remaining population and thus determine cell viability. The CV staining is directly proportional to the cell biomass and can be measured at 570 nm. CV staining is a quick and versatile assay for screening cell viability under diverse stimulation or inhibition conditions. BioVision’s CV cell cytotoxicity assay kit is simple, accurate, reproducible and sensitive. It also includes Doxorubicin as a positive control. This kit offers an excellent and efficient method for in vitro cytotoxicity studies as well as high-throughput drug screening


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Can you please let me know what the Solubilization Solution is and what is the purpose of using it? Is it necessary for the signal detection? Can we still measure the signal if we skip adding the Solubilization Solution?
The exact name and composition of the Solubilization Solution is proprietary and can’t be disclosed. However, once the cells are stained, the solubilization solution is added to re-solubilize the stained crystal violet, because you are drying the plates after crytal violet staining. This helps in more accurate measurement using spectrophotometry. Therefore, it is recommended to add the Solubilization Solution before measuring the OD.