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CRY1 (Human) ELISA Kit

A Sandwich ELISA kit for quantitative measurement of CRY1 in human serum, plasma, tissue lysates, and other biological fluids

WARNING: This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.
Catalog #: E4994
$750.00

Product Details

Cat # +Size E4994-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications Sandwich ELISA kit to quantitatively measure CRY1 in human serum, plasma, tissue lysate, and other biological fluids
Features & Benefits ● This Sandwich ELISA is highly sensitive and highly specific for the detection of CRY1 in human samples. There is no significant cross-reactivity or interference between CRY1 and analogues
● Detection range: 0.313 – 20 ng/ml
● Sensitivity: 0.188 ng/ml
● Recovery range: 85 - 105% for normal human serum and plasma samples
● Assay Precision: Intra-Assay CV and Inter-Assay CV < 10%
Kit Components ● Micro ELISA plate
● Wash Buffer (25X)
● Plate Sealers
● Standard (Lyophilized) (20 ng)
● Sample/Standard Dilution Buffer
● Biotin-labeled Antibody
● Antibody Dilution Buffer
● HRP-Streptavidin Conjugate (SABC)
● SABC Dilution Buffer
● TMB Substrate Solution
● Stop Solution
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Cryptochrome 1 (CRY1) is a photolyase-like-blue light receptor that belongs to the class of flavoproteins. It was first identified in Arabidopsis and later discovered in other lineages. In Arabidopsis, CRY1 mediates inhibition of hypocotyl elongation in response to blue light and photo periodically control floral initiation. In Drosophila, CRY1 binds to TIM in a light-dependent manner and mediates its degradation. In mammals, CRY1 heterodimerizes with PERIOD proteins, enters the nucleus, and suppresses CLOCK-BMAL1 transcriptional activity in a light-independent manner, thereby forming a negative feedback loop of the mammalian circadian clock. Additional functions of CRY1 include the regulation of glucose homeostasis and proinflammatory cytokine production. CRY1 is also known to downregulate the cAMP/PKA signaling pathway and promote cell growth and proliferation. Overexpression of CRY1 is associated with chronic lymphocytic leukemia and colorectal cancers and thus may serve as a promising target for anticancer therapy. BioVision’s CRY1 (Human) ELISA kit quantitatively measures CRY1 in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. HRP-Streptavidin is added to the plate, and post-incubation any unattached conjugates are washed off by Wash Buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of CRY1 in the sample or standard.


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