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Creatinine Colorimetric/Fluorometric Assay Kit

based on 19 citations in multiple journalsCreatinine Colorimetric/Fluorometric Assay Kit195 5
Catalog #: K625

In stock

$355.00

Product Details

Cat # +Size K625-100
Size 100 assays
Detection Method Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
Species Reactivity Mammalian
Applications N/A
Features & Benefits • Simple procedure; takes ~1 hour
• Fast and convenient
• Kit contains all necessary reagents for accurate measurement of Creatinine
Kit Components • Creatinine Assay Buffer
• Creatinine Probe
• Creatinase
• Creatininase
• Creatinine Enzyme Mix
• Creatinine (10 µmol)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Creatinine is a breakdown product of creatine phosphate. Creatinine is produced and excreted at a constant rate, and blood creatinine is used to determine glomerular filtration rate (GFR), a measure of kidney function. Blood creatinine levels increase only in cases of significant (>75%) damage to nephrons. Creatinine clearance is frequently used as a means of standardizing excretion of other compounds such as isoprostanes. BioVision’s Creatinine Assay Kit provides an accurate, convenient measure of creatine concentration in biological fluids such as serum, urine or CSF. In the assay, creatinine is converted to creatine by creatininase, creatine is converted to sarcosine, which is specifically oxidized to produce a product which reacts with a probe to generate red color (λmax = 570 nm) and fluorescence (Ex/Em = 538/587 nm). Unlike the picric acid assay, this kit is suitable for serum/plasma creatinine determinations, as well as for urine and other biological samples.


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What is the relation between lipid peroxidase and creatinine?
Creatinine is not directly related to lipid peroxidation. Isoprostane is used as a measure of lipid peroxidation. Creatinine is used for normalizing the Isoprostane concentration measured in different samples. Isoprostane/Creatinine ratio can be used as a measure for oxidative stress in samples.
If the standards turned brown instead of the usual purple color what is wrong?
The brown color reflects too much standard is used and the absorbance/fluorescence detector is saturated. Since the fluorometric assa is at least 10 times more sensitive, diluting the standard 1:100 as described in the datasheet should help resolve this issue. Also, the sensitivity of the fluorometer should be set at medium/low so that the detector does not get saturated easily.
Many samples read higher than the highest standard value in the fluorometric assay. 0.4ul of the probe was used but it was not diluted.
The fluorometric assay is at least 10x more sensitive than the colorimetric assay. Is it essential to dilute the probe 10x and then use 0.4ul of it to ensure the readings are not too high.
Can Creatinine levels from the same animal (rat) vary >10% in two different assays? Is this a stability issue?
Urine Creatinine is a very stable analyte. It is not known to aggregate like proteins can under storage. See: http://www.clinchem.org/content/44/8/1759.full If the diet of this animal was changed or there was oxidative stress or starvation, this could change urine creatinine concentrations from the same animal. Also, pipetting/dilution errors can account for differences.
Can EDTA or Citrate used to prepare blood samples interfere in the assay?
Citrate should fine. But EDTA being a metal chelator could interfere in the function of the enzymes used for detection in this assay. We do not recommend EDTA for blood collection for any enzyme-based detection assay.
Can individual components of this kit be purchased separately?
Yes, any of the kit's components can be purchased separately without having to buy the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Is it essential to make a standard curve for every expt, or is one curve/kit enough?
Yes, it is strongly recommended to do the standards every time you do the expt. There is always a chance that something was done differently that day and we do not want any conditions to differ between standards and samples.
Can frozen samples be used with this assay?
Fresh samples are always preferred over frozen samples. However, frozen samples can also be used, provided, they were frozen right after isolation, were not freeze thawed multiple time (for which we recommend aliquoting the samples before freezing) and have been frozen for relatively short periods.
Are trial sizes of this kit available?
Unfortunately, we do not have trial sizes of this kit available. However,for customers based in the US or Canada, a 10% off list price introductory discount can be offered on the list price. For all other customers based out of this area our regional BioVision distributor should be contacted.
How are samples normalized against protein concentration?
A protein quantitation assay can be used with the supernatants from cell/tissue lysates or with any other liquid sample in the assay buffer.
Alenzi, Mohammed et al. (2017) Antiurolithic effect of olive oil in a mouse model of ethylene glycol-induced urolithiasis, Investig Clin Urol. 2017 May;58(3):210-216.
Zhang, Lu et al. (2017) Nerolidol Protects Against LPS-induced Acute Kidney Injury via Inhibiting TLR4/NF-κB Signaling, Phytother Res. 2017 Mar;31(3):459-465.
Yao et al., Evaluation of urine fibrinogen level in a murine model of contrast-induced nephropathy. Vascular, Jun 2015; 10.1177/1708538115593039.
Burris et al., Estrogen directly and specifically downregulates NaPi-IIa through the activation of both estrogen receptor isoforms (ERα and ERβ) in rat kidney proximal tubule. Am J Physiol Renal Physiol, Mar 2015; 308: F522 - F534.
Wang et al., Astragaloside IV inhibits renal tubulointerstitial fibrosis by blocking TGF-β/Smad signaling pathway in vivo and in vitro.Experimental Biology and Medicine, Oct 2014; 239: 1310 - 1324.
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