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Creatine Colorimetric/Fluorometric Assay Kit

based on 2 citations in multiple journalsCreatine Colorimetric/Fluorometric Assay Kit24.1 4
Catalog #: K635

In stock

$365.00

Product Details

Cat # +Size K635-100
Size 100 assays
Detection Method Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
Species Reactivity Mammalian
Applications The kit can detect 0.001 – 10 mM Creatine.
Features & Benefits • Simple procedure; takes ~ 1 hour
• Fast and convenient
• Kit contains all necessary reagents for accurate measurement of Creatine
Kit Components • Creatine Assay Buffer
• Creatine Probe
• DMSO (Anhydrous)
• Creatine Enzyme mix
• Creatine Standard (10 µmol)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Creatine is an endogenous compound whose function is to maintain a high ATP/ADP ratio, by way of its phosphorylated form and creatine kinase. Creatine supplementation has been used in the treatment of muscular, neurological and neurodegenerative diseases, as well as a sport performance enhancer. Detection of creatine level has wide applications in research and development. BioVision’s Creatine Assay Kit provides an accurate, convenient measure of creatine in a variety of biological samples. In the assay, creatine is enzymatically converted to sarcosine which is then specifically oxidized to generate a product that converts a colorless probe to an intensely red color (λmax = 570 nm), and highly fluorescent (Ex/Em = 538/587 nm) product. Creatine is therefore easily detected by either colorimetric or fluorometric methods. Detection range 0.001 – 10 mM Creatine.


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All the standards and the samples, including ones without creatine turned dark pink. What could be wrong?
If the probe was exposed probe exposed to light/air it might get oxidized and yield color irrespectively.
Is it known if pyruvic acid, glucose, albumin, bilirubin and ascorbic acid interfere in this assay?
We are not aware of any interference by pyruvic acid, glucose, albumin, bilirubin and ascorbic acid. There might be cause-effect relationship between the creatine level in the sample and the concentration of these moieties but there is no reported interference with this assay per se that we are aware of. Sarcosine causes interference in this assay. The interference can be resolved by running side by side controls and subtracting the value from the results.
If there is no significant background, is it necessary to run background control for every sample?
Sarcosine creates background in this assay. If there is no significant background seen suring the pilot assay, it is not essential to run background controls for every sample.
Can frozen samples be used with this assay?
Fresh samples are always preferred over frozen samples. However, frozen samples can also be used, provided, they were frozen right after isolation, were not freeze thawed multiple time (for which we recommend aliquoting the samples before freezing) and have been frozen for relatively short periods.
Is it possible to use a different wavelength than recommended for the final analysis?
It is always recommended to use the exact recommended wavelength for the most efficient results. However, most plate readers have flexibility in their band width of detection in increments of +/- 10 nm. Depending on this flexibility range, you can deviate from the recommended wavelengths within limits.
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. It is recommended to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Are trial sizes of this kit available?
Unfortunately, we do not have trial sizes of this kit available. However,for customers based in the US or Canada, a 10% off list price introductory discount can be offered on the list price. For all other customers based out of this area our regional BioVision distributor should be contacted.
What is the shelf life of this kit?
This kit is good for 12 months from the date of shipment in the unopened form when stored at the appropriate temperature and appropriate conditions. After opening and reconstitution, some of the components in this kit are good for 2 months at -20°C. Please refer to the datasheet for storage information and shelf life of each of the components.
Jeannette Abplanalp et al., The cataract and glucosuria associated monocarboxylate transporter MCT12 is a new creatine transporter. Hum. Mol. Genet., Apr 2013; 10.1093/hmg/ddt175.
Joely A. Straseski, et. al. Investigating Interferences of a Whole-Blood Point-of-Care Creatinine Analyzer: Comparison to Plasma Enzymatic and Definitive Creatinine Methods in an Acute-Care Setting. Clin. Chem., Nov 2011; 57: 1566 - 1573.
For more citations of this product click here