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CPT1A (Human) ELISA Kit

A Sandwich ELISA kit for quantitative measurement of CPT1A in human serum, plasma, tissue lysates, and other biological fluids
Catalog #: E4997
$795.00

Product Details

Cat # +Size E4997-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications Sandwich ELISA kit for quantitative measurement of CPT1A in serum, plasma, cell and tissue lysates, and other biological fluids
Features & Benefits ● This Sandwich ELISA is highly sensitive and highly specific for the detection of CPT1A in human samples. There is no significant cross-reactivity or interference between CPT1A and analogues
● Recovery range: 85 - 105% for normal human serum and plasma samples
● Assay Precision: Intra-Assay CV and Inter-Assay CV < 10%
● Detection range: 15.625 – 1000 pg/ml
● Sensitivity: 9.375 pg/ml
Kit Components ● Micro ELISA plate
● Wash Buffer (25X)
● Plate sealers
● Standard (Lyophilized) (1000 pg)
● Sample/Standard Dilution Buffer
● Biotin-labeled Antibody
● Antibody Dilution Buffer
● HRP-Streptavidin Conjugate (SABC)
● SABC Dilution Buffer
● TMB Substrate Solution
● Stop Solution
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Carnitine palmitoyltransferase I (CPT1A) is an integral membrane protein that plays a significant role in the beta-oxidation of fatty acids. This liver-specific isoform of CPT is present on the outer membrane of the mitochondria of all cells except skeletal muscle cells and brown adipose cells. Unlike short-chain and medium-chain fatty acyl CoAs, long-chain fatty acyl CoAs cannot pass through the inner mitochondrial membrane. CPT1A converts these long-chain acyl CoAs to acylcarnitines, thereby enabling them to shuttle through the inner mitochondrial membrane. These acylcarnitines are re-converted to long-chain acyl CoAs in the mitochondrial matrix and undergo further metabolization to produce energy. CPT1A is less sensitive to malonyl CoA-mediated inhibition compared to other isoforms (CPT1B and CPT1C). Mutations of CPT1A lead to a rare metabolic disorder called Carnitine Palmitoyltransferase I deficiency. CPT1A is also associated with tumor development in multiple cancers such as prostate, breast, and lymphocytic leukemia. Therefore CPT1A could be a promising target to develop therapeutic agents to treat metabolic diseases and cancer. BioVision’s CPT1A (Human) ELISA kit quantitatively measures CPT1A in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. HRP-Streptavidin is added to the plate, and post-incubation any unattached conjugates are washed off by Wash Buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of CPT1A in the sample or standard.


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