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Coronavirus (SARS-CoV-2) PCR Detection Kit

qPCR based detection kit
Catalog #: K1460
$495.00

Product Details

Cat # +Size K1460-100
Size 100 Rxns
Detection Method qPCR based detection kit
Applications An ideal tool to detect SARS-CoV-2 by RT-PCR method
Features & Benefits • Inclusivity: SARS like coronavirus and specific detection of SARS-CoV-2
• Rapid, reliable amplification and detection
• Includes positive and negative control
• Dual Color Multiplex Assay Format
Kit Components • 2X qPCR Master Mix
• Reverse Transcription Mix
• PCR Primer/ Probe set
• Rehydration Buffer
• COVID-19 Positive control (PTC)
• Non-Template Negative Control (NTC)
Storage Conditions -20ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

BioVision’s SARS-CoV-2 Coronavirus PCR-Detection Kit is used for in vitro detection of SARS-CoV-2 in respiratory specimens using Real-Time (RT-PCR). The coronavirus SARS-CoV-2 was announced as the etiological agent of cases of ongoing pneumonia outbreak in Wuhan City. BioVision’s Coronavirus Detection Kit allows efficient cDNA synthesis and RT- PCR in a single tube. This kit includes a qPCR master mix that contains all the reagents supplied in a 2X concentration to perform RT- PCR. A separate Reverse Transcription mix that comprises a balanced mixture of both RTase (Reverse Transcriptase) and RNase Inhibitor is provided. In addition, the kit contains one set of primers and fluorescent probes to differentiate between SARS-CoV-2 and SARS-CoV (or bat SARS related CoV). The probes are read in different channels. Coronavirus SARS-CoV-2 RNA targets are amplified and detected in the FAM channel, Coronavirus SARS-CoV-2, SARS-CoV (or bat SARS related CoV) RNA targets are amplified and detected in the HEX, VIC or JOE channel (depending on the equipment used). This kit can be used to detect SARS-CoV-2 in respiratory specimens such as sputum, nasopharyngeal, oropharyngeal aspirates, washes or swabs and tracheal aspirates. BioVision offers the PCR Detection Kit to facilitate research on Coronavirus and improve patient treatment.


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Does the Kit specifically detect Novel Coronavirus 2019 (SARS-CoV-2)?
Yes, the kit specifically detects SARS-CoV-2. A specific probe for detecting SARS-CoV-2 is provided in the kit.
Does the kit detect two strains of Coronavirus in two fluorescent channels in QPCR? Can I detect SARS-CoV-2 in FAM channel and SARS-CoV in the HEX, VIC, JOE channel?
The kit contains 2 probes: One of the probes specifically detects SARS-CoV-2 RNA transcript in the FAM Channel. But it does not detect SARS-CoV RNA in the FAM Channel. The other probe detects SARS-CoV-2, SARS-CoV and bat-SARS-related CoVs in the HEX/VIC/JOE channel. The assay must be positive in both FAM and HEX channels for the detection of SARS-CoV-2.
What is the Positive Control used in the Coronavirus (SARS-CoV-2) PCR Detection Kit, Cat# K1460?
The Positive Control used in the Coronavirus (SARS-CoV-2) PCR Detection Kit, Cat# K1460 is a plasmid containing a part of the 2019-nCoV RdRP gene. This is to make sure that the kit components are all working.