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Coronavirus Rapid RT-qPCR Detection Kit

qPCR based detection kit to detect SARS-CoV-2
Catalog #: K1461

Product Details

Cat # +Size K1461-100
Size 100 rxns
Detection Method qPCR based detection kit
Applications • An ideal tool to detect Coronovirus SARS-CoV-2 by RT-PCR method
Features & Benefits • Compatible with standard RT-qPCR machines (Bio-Rad CFX96, QuantStudio’s 7 Flex system)
• Highly specific for the ORF1ab and N target markers recommended by WHO and US CDC
• Reliable and Ready-to-use
• Includes a Positive Control
• Results ready in less than 2 hr
Kit Components • COVID-19 Primers/Probes
• 2X RT-qPCR Master Mix
• Positive Control Template
• Negative Extraction Control
• RT-qPCR Enzyme Mix 40 μl
• Nuclease-free Water 1 ml
Storage Conditions -20ºC
Shipping Conditions Dry Ice
USAGE For Research Use Only! Not For Use in Humans.


BioVision’s Coronavirus rapid RT-qPCR Detection Kit is used for the detection of SARS-CoV-2 in respiratory specimens using Reverse Transcriptase Quantitative Polymerase Chain Reaction (RT-qPCR). It allows the efficient cDNA synthesis and qPCR in a single tube. This kit includes a 2X RT-qPCR Mastermix that contains all the reagents supplied in a 2X concentration to perform the qPCR. A separate RT-qPCR Enzyme Mix for cDNA synthesis is included the kit. Additionally, the kit contains 2 vials of primers and probes specific for the ORF1ab and N target. The PCR Detection Kit will facilitate the Coronavirus research and improve patient treatment.

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Is the Primer and Probe for the internal control RP provided in the Kit?
Yes, the Primer and Probe for the internal control RP are included in the COVID-19 Primers/Probes, K1461-100-1.
What is the bp size of the amplicon for the RNA-dependent RNA polymerase (RdRP), nucleocapsid (N) and RP targets?
The Coronavirus Rapid RT-qPCR Detection kit is specific for the RNA-dependent RNA polymerase (RdRP) and nucleocapsid (N). The amplicon sizes for these proteins and the internal control, RP are given below:
N2: 67 bp
RdRP: 108 bp
RP: 65 bp
What is the Positive Control provided in the Kit?
The Positive Control is a DNA plasmid containing the sequences of RNA-dependent RNA polymerase (RdRP) and nucleocapsid (N). The Positive Control is provided to make sure that the kit components are all working.