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Product Details
| Kit Summary | A detection card used for the qualitative in vitro detection of IgM and IgG antibodies against the novel Coronavirus (Sars-Cov-2). |
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| Detection Method | Detection of IgM and IgG antibodies against the novel Coronavirus (Sars-Cov-2) |
| Applications | • A rapid tool to detect Coronavirus IgG and IgM antibodies |
| Features & Benefits | • Direct observation without any instruments • Rapid, easy and convenient • Obtain results in 3-15 min • High sensitivity and specificity |
| Kit Components | • Test Card • Sample Diluent |
| Storage Conditions | 4°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
BioVision’s Coronavirus (SARS-CoV-2) IgM/IgG Antibody Detection Card is used for the qualitative, in vitro detection of IgM and IgG antibodies against the novel Coronavirus (Sars-Cov-2) in human serum, plasma, whole blood or fingertip blood. The detection is based on the colloidal gold immune-technology for detecting novel Coronavirus nucleoprotein (N)/spike glycoprotein (S) IgM or IgG antibodies. The Test Card contains a nitrocellulose membrane and a combination card. The detection area of the nitrocellulose membrane is coated with mouse anti-human IgG and mouse anti-human IgM antibodies to detect the novel Coronavirus nucleoprotein (N)/spike glycoprotein (S) antibodies and the control area is coated with rabbit anti-chicken IgY. The combination card comprises of sprayed colloidal gold labeled recombinant novel Coronavirus nucleoprotein (N)/spike glycoprotein (S) antigen and chicken IgY, sample pad, absorbent pad and a PVC soleplate. If the sample contains the novel Coronavirus nucleoprotein (N)/spike glycoprotein (S) IgM or IgG antibodies, these antibodies will bind to the gold labeled virus antigen. The binding of antibodies in the sample to the antigen forms a sandwich immune complex with the coated anti-human IgM and IgG monoclonal antibodies, which develops a purplish red color indicating that the novel Coronavirus nucleoprotein (N)/spike glycoprotein (S) antibodies are present in the sample. BioVision offers the IgM/IgG Antibody Detection Card for researchers to quickly detect Coronavirus in samples and aid in research on Coronavirus.
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BioVision aims to provide our customers innovative tools for accelerating drug discovery and biological research. BioVision offers >8,000 products including the most comprehensive array of assay kits for key targets in Metabolic pathways. |
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What is the principle behind the use of Antibody Detection Card for Coronovirus?
The Coronavirus (SARS-CoV-2) IgM/IgG Antibody Detection Card detects IgM and IgG antibodies against the novel Coronavirus (Sars-Cov-2) based on the principle of lateral flow immunochromatography using colloidal gold immune-technology. When the sample is added to the sample hole of the Test Card, the sample will move along the detection card by chromatography. If the sample contains novel Coronavirus nucleoprotein (N)/spike glycoprotein (S) IgM or IgG antibodies, the antibodies will bind to the gold labeled novel Coronavirus antigen in the card. This binding to the antigen will form an immune sandwich complex with the coated anti-human IgM or IgG monoclonal antibody at the M or G line. The immune complex develops a purplish red color at the M or G line, which indicates the presence of novel Coronavirus nucleoprotein (N)/spike glycoprotein (S) IgM or IgG antibodies in the sample.
What is the Sample Diluent provided in the Kit?
The Sample Diluent is a Phosphate Buffer Solution (pH 6.5-8.0).
Should the samples be diluted before performing the test?
The end-user can directly add blood samples to the detection card, followed by the diluent.
What is the sample dilution ratio for kit? Do I need to determine the sample dilution?
The dilution factor is about 3-6 times. The end-user can mix one drop of blood with two drops of diluent for the test.
