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MMP-1 (Human) ELISA Kit

A Sandwich ELISA kit for the quantitative measurement of MMP-1 in human serum, plasma, culture supernatants and other biological fluids.

WARNING: This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California to cause cancer.  For more information go to  www.P65Warnings.ca.gov.
Catalog #: E4646
$715.00

Product Details

Cat # +Size E4646-100
Size 100 assays
Kit Summary • Detection method- Absorbance (450 nm)
• Species reactivity- Human
• Application- quantitative measurement of Collagenase I in serum, plasma, tissue homogenates and other biological fluids.
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications This ELISA kit is used for quantitative measurement of MMP-1 in serum, plasma, tissue homogenates and other biological fluids.
Features & Benefits • Assay Precision: Intra-Assay CV < 8% and Inter-Assay CV < 10%
• Detection range: 78.125 - 5000 pg/ml
• Sensitivity: 46.875 pg/ml
• Recovery range: 85 - 105% for normal human serum and plasma samples
• This Sandwich ELISA is highly sensitive and highly specific for the detection of MMP-1 in human samples. There is no significant cross-reactivity or interference between MMP-1 and analogues
Kit Components • Micro ELISA plate
• Standard (Lyophilized) (5000 pg)
• Sample/Standard Dilution Buffer
• Biotin-labeled Antibody
• Antibody Dilution Buffer
• HRP-Streptavidin Conjugate (SABC)
• SABC Dilution Buffer
• TMB Substrate Solution
• Stop Solution
• Wash Buffer (25X)
• Plate Sealers
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Matrix metalloproteinase-1 (MMP-1), also known as collagenase-1, fibroblast collagenase, and interstitial collagenase belong to the family of matrix metalloproteinases (MMPs). Cells that express MMP-1 include macrophages, monocytes, keratinocytes, and fibroblasts. These endopeptidases can breakdown extracellular matrices as well as interstitial collagen types I, II, III. MMP-1 plays a significant role in diseases such as cancer, rheumatoid arthritis, fibrotic and cardiovascular diseases and thus could serve as a therapeutic target in the treatment of human diseases. BioVision’s MMP-1 (Human) ELISA kit is based on the Sandwich ELISA principle to quantitatively measure the amount of MMP-1 in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. The HRP-Streptavidin is added and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of MMP-1 in the sample or standard.


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