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Collagenase (Collagen Degradation/Zymography) Assay Kit (Fluorometric)

Sensitive Fluorometric Assay to Detect Collagenase in Mammalian Samples
Catalog #: K490

Product Details

Cat # +Size K490-100
Size 100 assays
Kit Summary • Detection method- Fluorometric (490/520 nm)
• Species reactivity- mammalian
• Applications- - Measurement of collagenase activity
- For screening/studying/characterizing collagenase inhibitors
Detection Method Fluorescence (490/520 nm)
Species Reactivity Mammalian
Applications • Measurement of collagenase activity
• For screening/studying/characterizing collagenase inhibitors
Features & Benefits • Rapid & sensitive
• Simple assay to measure collagenase activity as well as to screen/study/characterize potential inhibitors of collagenase
• Includes Fluorometric Collagenase Substrate and Positive Control
Kit Components • Collagenase Assay Buffer
• Cell Lysis Buffer
• Enzyme Positive Control
• Collagenase (Substrate)
• FITC Standard (5 mM)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Collagenases, members of the matrix metalloproteinase (MMP) family, are endopeptidases that digest native collagen in the triple helix region and are involved in the physiological and pathological turnover of connective tissues. Collagens are the major fibrous component of animal extracellular connective tissue. Bacterial collagenases differ from vertebrate collagenases in that they exhibit broader substrate specificity. Collagenases may play a role in facilitating tumor cell invasion of the extracellular matrix at multiple stages of the metastatic process. Collagenases have also been approved for medical uses for the treatment of Dupuytren's contracture, Peyronie's disease and wound healing. Collagenase activity is usually detected by small peptide-based activity assays which may suffer from lack of substrate specificity. Other methods for collagenase activity include collagen Zymography where samples are electrophoresed on a collagen-containing SDS-PAGE, and further renatured in a suitable buffer for 12-16 h. The zymogram is subsequently stained, and areas of digestion appear as clear bands against a darkly stained background where the substrate has been degraded by the enzyme. Such methods are laborious, time-consuming and may lead to the loss of enzymatic activity as renaturation may not be completely reversible. BioVision’s Collagenase Activity Assay Kit utilizes a hybrid approach for the detection of collagenase activity by employing a highly quenched collagen substrate which upon cleavage by a suitable collagenase releases a fluorophore, which can be easily quantified using a conventional microplate reader. This method is substrate-specific, simple, fast, high-throughput adaptable and amenable to the sensitive detection of collagenase activity (as low as 0.6 mCDU for bacterial collagenase) in biological samples

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What does an mCDU unit mean?
There are different units to express collagenase activity. In K490, 1 CDU is the amount of Collagenase required to cleave the Collagenase Substrate and release 1 pmol of Fluorescein per min under the conditions of the assay.