Collagenase Activity Colorimetric Assay Kit

Catalog #: K792 | abID: ab196999

Product Details

abID ab196999
Cat # +Size K792-100
Size 100 assays
Detection Method Absorbance (345 nm)
Species Reactivity Bacterial
Applications Measurement of collagenase activity
For screening/studying/characterizing collagenase inhibitors
Features & Benefits • Rapid & sensitive
• Simple assay to measure collagenase activity as well as to screen/study/characterize potential inhibitors of collagenase
• Includes Inhibitor Control (1,10-Phenanthroline)
Kit Components • Collagenase Assay Buffer
• Collagenase
• Collagenase Substrate (FALGPA)
• Inhibitor (1 M 1,10-Phenanthroline)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Collagenase (EC 3.4.24.3) is an enzyme in the matrix metalloproteinase family that breaks down collagen, assisting in degradation of the extracellular matrix, a key step in the pathogenesis of bacteria. Collagen is an abundant structural protein present in the connective tissue of animals. Collagenase has been used clinically for the treatment of Dupuytren’s contracture, an affliction characterized by a thickening of connective tissue. BioVision’s Collagenase Activity Assay Kit provides a quick and easy way to determine activity of Collagenase. The Kit measures collagenase activity using a synthetic peptide (FALGPA) that mimics collagen’s structure. It is suitable for measuring activity of bacterial collagenases such as from Clostridium histolyticum type I-XI. In addition, it can also be used to screen/characterize collagenase inhibitors. The limit of detection for this assay is 0.02 mU Collagenase.


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Why is the variable “time = ΔT” in minutes missing from the final units?
Activity of collagenase is presented in U/ml. U = substrate/min. Therefore, time is already included in the final units, U/ml.
If we want to measure activity in Wuensch Units, how is FALGPA related to WU?
Below is the unit definition for FALGPA and Wünsch units. One FALGPA Unit catalyzes the hydrolysis of 1 µmole of N-(3-[2-furyl]-acryloyl)- L- leucylglycyl- Lprolyl-L-alanine (FALGPA) per minute at 25°C, pH 7.5. One Wünsch units(PZ) unit catalyzes the hydrolysis of 1 µmole 4-phenylazobenzyloxycarbonyl- L-prolyl- Lleucyl-glycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1. Approximately speaking,1 Wünsch unit = ~3.9 FALGPA units