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Coelenterazine, native

A luminescent substrate
Catalog #: 2536
SKU-Size Size Price Qty
2536-500 500 µg
2536-1000 1 mg
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Product Details

Alternate Name 3,2-Dihydro-2-(p-hydroxybenzyl)-6-(p-hydroxyphenyl)-8-benzylimidazolo[1,2-a]pyrazin-3-one, CLZN
Appearance Brownish yellow solid
CAS # 55779-48-1
Molecular Formula C₂₆H₂₁N₃O₃
Molecular Weight 423.46
Purity ≥95% by TLC
Solubility EtOH or MeOH
InChi InChI=1S/C26H21N3O3/c30-20-10-6-18(7-11-20)15-23-26(32)29-16-24(19-8-12-21(31)13-9-19)27-22(25(29)28-23)14-17-4-2-1-3-5-17/h1-13,16,27,30-31H,14-15H2
PubChem CID 2830
MDL Number MFCD00467176
Handling Protect from air and light
Storage Conditions -20 ̊C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Cell-permeable. Coelenterazine is the luminescent substrate for Renilla luciferase and apoaequorin. When coelenterazine combines with luciferase, oxygen, and calcium ions, it produces oxy-coelenterazine and light. This allows coelenterazine to be used for bioluminescent calcium detection and the monitoring of reporter genes in BRET (Bioluminescence Resonance Energy Transfer), ELISA and HTS methods as well as chemiluminescent detection of superoxide anions and peroxynitrite in cells or tissues.

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