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New

Choline Kinase (ChoK) Activity Assay Kit (Fluorometric)

Sensitive Assay, HTS
Catalog #: K476

In stock

$475.00

Product Details

Cat # +Size K476-100
Size 100 assays
Kit Summary • Detection method- Fluorescence (Ex/Em 535/587 nm)
• Species reactivity- Eukaryotes
• Applications- The kit detects 0.5 µM choline kinase.
Detection Method Fluorescence (Ex/Em= 535/587 nm)
Species Reactivity Eukaryotes
Applications Measurement of choline kinase activity in different cell lines and tissues
Features & Benefits • Simple procedure; takes ~ less than 40 minutes
• Fast and convenient
Kit Components • Assay Buffer
• Substrate
• Detection Mix I
• Detection Mix II
• ATP
• Probe
• ADP Standard (1 µmol)
• Human ChoK
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Pyruvate kinase (PK, EC 2.7.1.40) is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. Lack of pyruvate kinase will slow down the process of glycolysis which causes the disease known as pyruvate kinase deficiency. BioVision provides a simple, direct and automation-ready procedure for measuring pyruvate kinase activity in various biological samples such as blood, tissues, and culture cells, etc. In the assay, PEP and ADP were catalyzed by PK to generate pyruvate and ATP. The generated pyruvate is oxidized by pyruvate oxidase to produce color (at λ = 570 nm) and fluorescence (at Ex/Em = 535/587 nm). Since the increase in color or fluorescence intensity is proportional to the increase in pyruvate amount, the PK activity can be accurately measured. The kit detects 0.1 mU/ml pyruvate kinase.


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