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Chlortetracycline ELISA Kit

A Competitive ELISA kit for in vitro quantitative determination of Chlortetracycline concentrations in Tissue, Liver, Eggs, Honey, Urine.

WARNING: This product can expose you to chemicals including TMB, which is [are] known to the State of California to cause cancer.  For more information go to
Catalog #: E4782

Product Details

Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Universal
Applications in vitro quantitative determination of Chlortetracycline concentrations in Tissue, Liver, Eggs, Honey, Urine
Features & Benefits • Sample recovery rate: Tissue, Liver, Eggs -90%±20%; Honey -75%±20%; Urine -80±20%
• Detection range: Tissue, Liver, Eggs - 0.8 ppb, Honey - 4 ppb, Urine -1 ppb
• Sensitivity: 0.1 ppb
• Cross-reactivity: Chlortetracycline - 100%, Tetracycline -29%, Oxytetracycline -15%, Doxycycline -2.5%
Kit Components • Standard (1.0 ppm)
• Reconstitution Buffer (5X)
• ELISA plate
• Substrate Reagent A
• Wash Buffer (20X)
• Substrate Reagent B
• Antibody Working Solution
• Plate Sealer
• HRP Conjugate
• Stop Solution
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Chlortetracycline is an antibiotic produced by some strains of Streptomyces aureofaciens. It inhibits protein synthesis (elongation) by preventing binding of aminoacyl-tRNA to the 30S subunit. It is effective against Gram-positive and to a lesser degree Gram-negative bacteria than tetracycline. BioVision Chlortetracycline ELISA Kit is based on Competitive ELISA principle. The micro-plate provided in this kit has been pre-coated with Chlortetracycline. During the reaction, Chlortetracycline in the samples or standard competes with Chlortetracycline coated on the plate for binding to the anti-Chlortetracycline antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of Chlortetracycline. The concentration of Chlortetracycline in the samples can be calculated by comparing the OD of the samples to the standard curve.

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