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Chlamydia Trachomatis IgA ELISA Kit

Chlamydia Trachomatis IgA ELISA KIt is intended for the determination of specific IgA antibody to Chlamydia in a single human serum sample.

WARNING: This product can expose you to chemicals including TMB, which is [are] known to the State of California to cause cancer.  For more information go to
Catalog #: E4711

Product Details

Cat # +Size E4711-100
Size 96 assays
Detection Method Absorbance-450 nm
Species Reactivity Human
Applications This ELISA kit is used for the determination of specific IgA antibody to Chlamydia in human serum samples
Features & Benefits • Accuracy (Overall agreement) = (A+D) / (A+B+C+D) = 38 / 40 = 95%
• Sensitivity = A / (A+B)= 5 / (5 + 0) = 100%
• Specificity = D / (C + D) = 33 / (33+2) = 94%
Kit Components • Microwell strips
• Sample Diluent
• Stop Solution
• Negative Control
• Positive Control
• Enzyme Conjugate
• Calibrator
• Wash Buffer (10X)
• TMB Chromogenic Substrate
Storage Conditions 2-8°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Chlamydia Trachomatis is one of the most common human pathogens. Of the 15 recognized serotypes, 4 (A, B, Ba, and C) have been shown to cause hyperendemic blinding trachoma, a disease which afflicts hundreds of millions of people in developing countries. Three serotypes (L-1, L-2, and L-3) are the causes of lymphogranuloma venereum (LGV), a sexually transmitted systemic disease.High levels of anti-Chlamydia IgG antibody are of diagnostic value in chronic or systemic infections such as salpingitis, mechanical infertility, perihepatitis, epididymitis, Reiter’s syndrome and pneumonitis. BioVision's Chlamydia Trachomatis ELISA Kit employs the LGV type 2 broadly reacting antigen of Chlamydia Trachomatis. It will detect Chlamydia Trachomatis, Chlamydia Psittaci and Chlamydia Pneumoniae (TWAR) antibodies. Purified Chlamydia Trachomatis antigen is coated on the surface of microwells. Diluted patient serum is added to wells, and the Chlamydia Trachomatis IgA specific antibody, if present, binds to the antigen. All unbound materials are washed away. After adding enzyme conjugate, it binds to the antibody-antigen complex. Excess enzyme conjugate is washed off, and TMB Chromogenic Substrate is added. The enzyme conjugate catalytic reaction is stopped at a specific time. The intensity of the color generated is proportional to the amount of IgA specific antibody in the sample. The results are read by a microwell reader compared in a parallel manner with calibrator and controls.

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